Tcm Triangular And Eupolyphaga Of The Polycystic Kidney Disease-lining Epithelial Cell Proliferation And Signal Transduction

Autosomal-dominant polycystic kidney disease is an inherited disorder usually manifest in adulthood and essentially characterized by the development of multiple renal cysts variably associated with extrarenal(mainly hepatic and cardiovascular) abnormalities. Traditional chinese medicine is an important method to treat ADPKID.To investigate the effect of chinese herb on autosomal dominant polycentric kidney disease(ADPKD) cystic-lining epithelial cells, we built up a culture method of primary culture of ADPKD cystic-lining epithelial cells in vitro and transfected the SV4O into primary cultured cells in order to establish a cell strain. Then, we observed the effect of epidermal growth factor(EGF), Compond 32(PTK inhibitor), Sparganium stoloniferum Buch Ham(SBH) and Eupolyphaga sinensis Walker(EW) on both phosphorylation of EGFR, mitogen-activated protein kinase(MAPK) and proliferation of cystic-lining epithelial cell sti1ain by Brdu in corporation assay, flow cytometer detect and w~鐂tern blot method. The resultS show: ~D there was no significant difference in the cell biological characteristics found between cell strain and primary cultured cells; ~ EGF with the concentration from 2.5 to 2Ong/ml had a stimulating effect on the cyst-lining epithelial cells compared with untreated control 2 98 ~ group(p < 0.01); ?Compond32(0.2?.Oumol/L) could significantly inhibit the prolifration of cyst-lining epithelial cells actived by EGF(lOng/ml) compared with treated with EGF(lOng/ml) only(p ~ 0.01); ?SBH and EW serum(1%?%) could significantly inhibit the prolifration of cyst-lining 昬pithelial cells actived by EGF(1 OngIml) compared with EGF(lOng/ml) plus untreated serum(1%~5%) group (p < 0.01);?Compond32, SBH and EW could inhibit phosphorylation of EGFR and MAPK in cyst-lining epithelial cells stimulated by EGF. The result suggest that ADPKD cyst-lining epithelial cell strain can be established by SV4O transfect, and the cell strain can be used as a kind of cell model in the research of ADPKD cellular and molecular mechamism; EGF maybe accelerate the ADPKD development; Compond 32,EW and SBH might have beneficial effects on prolong the progression of ADPKD.