The Shengmai To Bone Capsule Treatment Of Femoral Head Necrosis Of Bone Repair Mechanism

Avascular necrosis of the femoral head (ANFH) results from the blood supply impairment by various causative factors. Its basic pathologic change is the death of bone and marrow elements. At the same time, a process of repair then is initiated. The reparative reaction is late and ineffective in glueocorticoid-induced avascular necrosis of the femoral head (GANFH), which is involved extensive size and difficult to treat. Scholars in the world are looking for effective methods in improving the reparative process. GANFH belongs to blood stasis in TCM. Shengnai Chenggu Capsule is a recipe made by Prof. Yuan llao for the treatment on avascular necrosis of the femoral head. It is efficacious in activating circulation of the blood so as to eleminate blood stasis, tonifying the kidney and strengthening the bone, suppressing inflammation and alleviating pains. The long-term clinical practice has proved it is an effective medicine for ANFH. But its effect mechanism remains further research. GANFH model in rabbits was induced by injecting endotoxin and niethylprednisolone. We investigated the promotion effect mechanism of Shengmai Chenggu Capsule on GANFII. 56 New Zealand rabbits were randomly allocated into normal control group and the test. 38 rabbits in the test were given S0uglkg of endotoxin from Escherichia intrvenously twice at an interval of 24 hours, and injected intramuscularly with 20mg/kg of methylpreduisolone 3 times at intervals of 24 hours. 2 rabbits were sacrificed for ascertainment of GANFLI after two weeks. The left were randomly allocated into model group and treatment gr1u1 With Shengmai Chenggu Capsule. Rabbits in treatment group were respectively received Shengmai Chenggu Capsule 2.lg.k?d? Rabbits in normal and model control group were respectively received 2.lg.k?d?Saline. 6 rabbits were killed at the time of fourth week, eighth week, twelfth week in each group respectively. Bone gla protein (BGP) and interleukin-6 (IL-6) in serum were assayed. Results :1. By light microscopy, in model group empty lacunae increased. Quality of osteoblasts, MTW and TBV in model group decreased. Specimens in treatment group showed those pathologic changes relieved gradually along with active new bone formation. 2. By electron microscopy, in model group the function organeli in osteoblasts showed impaired severely. Small lipid droplets in osteocytes gradually, which resulted in cell death, in treatment group organeli in osteoblasts and osteocytes maintained intact structure. 3. In situ hybridization, it decreased expression of type I collagen messenger RNA in osteoblasts and osteoprogenitor cells in model group, whereas increased in treatment group. 4 By biochemistry analysis, it decreased the bone content of collagen, calcium and phosphorus in subchondral bone in model group. It increased in treatment group. 5. By radioimmunology analysis, level of BGP in sum gradually decreased in model group, whereas LL-6 increased. Level of BGP in serum gradually increased in treatment group, whereas IL-6 maintained normal level. In conclusion, decreased bone formation and increased bone loss awe close correlated to disease progression of GANFII. Shengn~qi Che~t鏶u Capsule is able to reverse disease progression With improving bone form~ion and inhibiting bone loss. This effect is more obvious in a time-dependent mann...