Cerebral Ischemia And Reperfusion Mitochondrial Rna Expression And Protein Synthesis And Intervention Factors

PURPOSES The ischemic injury of neurons happens in brain vascular diseases very commonly. It has a serious results. With the development of medical techniques,we have better methods to treat brain vascular diseases. The commom methods is to reperfuse . but reperfusion will results neurons injury . the injury will lead to bad results. So reperfusive injury always is the research hotspot of ischemic brain vascular diseases.After research, we found the major form of reperfusive injury is apoptosis. Apoptosis has two routes: 1 )death receptor route 2) mitochondria route.For the route of mitochondria.researchs mainly focus on the configuration changes and it's function in apoptosis.the mRNAs of gene coded by mitochondria have not been researched deeply.As the only cell organ possess DNA,the expression of gene coded by mitochondria is different with nucleus'.the difference perhaps decide the function of mitochondria during ischemia and reperfusion.the aim of our research is to focus on the expression of gene coded by mitochondria during ischemia and reperfusion.using the methods of Molecularbiology, we can examinate the expression of gene coded by mitochondria, at the same time ,we use interference factor to affect expressions of genes coded by mitochondria. With results we can explain the characteristics and gains and losses factor , study the new mechanism of apotosis during schemia and reperfusion in neurons, provide the basis of theories andmethods for the treatment to brain ischemic and reperfusive injure. . Method: using the methods of carotid shunt make models of rat brain ischemia and reperfusion,select the area of Hippocampus CA1 as research object. Establish the system of mitochondrial transcription and translation, using this system ,we research mitochondrial RNA synthesis and mitochondrial protein synthesis, explore the affection of ischemia and reperfusion to mitochondrial RNA synthesis and mitochondrial protein synthesis.2? using the methods of PCR ,examine the expression of gene cox I?II?III and ATPase6?ATPase9, explore the affection of ischemia and reperfusion to expression of gene coded by mitochondria 3? using high concentration ATP? high concentration adenosine and adenosine deaminase inhibitor? A2 receptor retarder?L-camitine and conjungated linolenic acid to interfere mitochondrial RNA synthesis and mitochondrial protein synthesis. using the methods of PCR examine the expressions of gene cox I?II?III and ATPase 6?ATPase9 after interference.from results ,we can know the affection of interference factor to expressions of genes coded by mitochondria RESULTS:1?Both of ischemia and reperfusion can affect mitochondrial transcription and translation, reperfusion can aggravate this injure.when we compare the affection of ischemia with reperfusion,we found the function of transcription descending 10.23%. the function of translation descending 16.85% duringischemia. The descent degree of translation is more serious than transcription's (P<0.05) The descent degree of translation and transcription almost is same during reperfusion.2.The ischemic injure undoubtly can affect the expressions of genes coded by mitochondria.and this affection has different effect to each gene. to Cox- I gene, ischemia can lower it's expression.reperfusion will aggravate this effect.to Cox-II gene, ischemia has no affection to it's expression.but reperfusion can ascend it's expression. expression of Cox D is stable during ischemia and reperfusion. |For the gene-ATPase6 , ischemia descend it's expression , reperfusion will aggravate this injure. ischemia and reperfusion have no the obvious influence to ATPase8 mRNA expression.3.With the high concentration ATP, The synthesis of RNA, protein have the further descent than reperfusion. the expressions of Cox I mRNA