| The theory of Traditional Chinese Medicine(TCM) Zangxiang is the core of TCM theory system.The TCM five visceras including form visceras and spirit visceras, on the basis of form visceras, we should know clearly the spirit visceras, namely the functional visceras.The theory of the correspondence between Five Zang and time is an important point in .The theory of correspondence between time and Zang means the corresponding change of the function of the body along with the change of environment. The five zang-organ is the modulating system of the human body.The action of five zang-organ in is the modulating function. Five zang-organ can not only modulate the body itself, but also modulate its correspondence with the nature.1 .ObjectiveOn the basis of "lung corresponding to autumn", we discuss the modulation mechanism of lung from the point of immunity, and the relative study of lung and immunity.We want to prove that the modulation of zang and fu-organs corresponding to time is scientific and can be repeated.2.Method2.1 Twenty-four SD male rats, 180-220g weight, three months age.We got it from Beijing Weitong Lihua animal study center forty days before vernal equinox and autumnal equinox, then divided into three groups randomly:normal group, fake group ans pinealectomy group.They were raised to take water and foods freely.Fake group and Px group were operated a month before vernal equinox and autumnal equinox, then on 8:00pm of vernal equinox and autumnal equinox, we should kill all the rats.2.2 Serum IgG was detected in the way of unilateralism immunity diffusion.Serum IL-2 was detected in the way of ezyme-linked immunosorbent assay.T lymphocyte translation rate of spleen was detected in MTT.IFN-yand IL-6 in bronchoalveolar lavage fluid were detected in ELISA and IL-10 and MT were detected in the way of radiation immunity analysis,etc.2.3 Data analysis method:All the data were showed in the form of x±s.The difference ofgroup was tested in SPSS 10.0.3.ResultsAll the substances including the immune materials and melatonin in normal group were low in autumn and high in spring, and index of spleen and thymus, T lymphocyte translation rate of spleen and IL-6 in BALF, etc.changed much.The difference is clear, p< 0.05.While pinealectomy, all the data in rats between spring and autumn didn't change much, and the substances all showed the trends of ascending, and some indexes change much compare to normal group.4.Conclusions(l)Pineal gland is one of the important medium in modulating mechanism of "lung correspondence with autumn".The results proved that melatonin in serum and lung is higher in spring and lower in autumn at normal state. When removed the pineal gland, the contents of melatonin in lung and serum increased as feedback, it has a clear difference compared with the normal group. The rhythm of melatonin higher in spring and lower in autumn is not evident while pinealectomy.The production of melatonin in serum and lung is not in the pineal gland while pinealectomy, it may be excreted from other organs, but the results connect with the stimulation of pinealectomy on neuroendocrine.We also found that the exponents of thymus gland and spleen, spleen T lymphocyte translation rate, IgG and IL-2 in serum, function ofmacrophage in pulmonary alveoli, IFN-Y and IL-6 in pulmonary alveoli fluid, IL-10 in lung tissue, etc. all showed the trends of higher in spring and lower in autumn in normal group, resembling the trends of melatonin in serum and lung tissue.Melatonin in serum has negative correlation with IgG in Spring Equinox, and melatonin in lung has a direct correlation with the function ofmacrophage in pulmonary alveoli in Autumn Equinox, which may give the reasons that rhythms of immunity in body and lung have an evident relationship with the modulation of melatonin in pineal gland. So we can say that pineal gland may be one of important medium in modulating the relationship between season and zang-fu organs.We affirmed that melatonin modulate the immunity of lung.(2)The theory of "lung correspondenc... |
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