Aplastic Anemia In Patients With Bone Marrow Mesenchymal Stem Cell Biological Characteristics And Adipogenic Differentiation Of Abnormal Studies,

Normal haemotopoiesis is sustained by interactions between stem cells and stroma. In aplastic anemia (AA) , the basic structure of the bone marrow (BM) becomes abnormal: cells responsible for generating blood cells (hematopoietic cells) are greatly decreased in number and are replaced by fat cells.Microenvironment of BM is constituted with stromal cells, adipocytes and osteoblasts, all derived from the mesenchymal stem cells (MSCs). PART ONETo further investigate the microenvironment of AA, we isolated and cultured the MSCs from AA patients and studied biologic characteristics of these cells.Under the same culture conditions, mononuclear cells from both AA patient and normal control were able to generate adherent MSCs, which showed a typical fibroblastlike structure. There is no evident difference in the morphology of MSCs between AA patient and control.Growth-pattern analysis showed that MSCs from AA patient proliferated slower than MSCs from control . FACS showed that MSCs from AA patient mainly expressed CD44, CD29, CD105, CD106 and FLK1, but not CD34, CD45, CD31, CD11a, CD11b or HLA-DR. Cell cycle analysis revealed that a small population of cells were actively engaged in proliferation (S+G2+M=3.49%), more than 90% of cells were in the G0/G1 phases. Under the appropriate culture conditions, MSCs from AA patient could differentiate...