The Aconitum Toxic Drugs Used On The Compatibility Of Drug Substances Basic Research, And

Objectives: Researching the effect on the contents of total alkaloids, ester alkaloids, alkaloids in the aconite root species and the active components(paeoniflorin,AQ, phenols) in the compatibility of others Chinese drugs and aconite root in the different compatibility ratio and circumstance, ascertaining the material basis of toxic reactions in the aconite root species, establishing the chemi-library of toxic or effective components in the different compatibility relationship, and approaching the sciencity and rationality of compatibility disciplines.Methods: Through different medical materials and different compatibility ratio in the circumstance of decoction,artificial gastric and intestinal juice, applying multi-indexes evaluation system to evaluate the compositions and components of toxicity and potency. Applying acid dye colorimetry and hydroxamic acid iron spectrophotometry to determine total alkaloids and ester alkaloids respectively in the double-drugs compatibility of aconite root species; using HPLC to determine the components of alkaloids in aconite root, paeoniflorin, AQ and phenols.Results: The content of total alkaloids shows step-up tendency, and the content of ester alkaloids shows step-up or descended tendency in the compatibility of Radix aconiti (CRA, RAP)and Radix Paeoniae Albae (RPA), Radix aconiti kusnezoffii (CRAK, RAKP)and RPA, RALP(CRALP, WPLRA, BPLRA) and RRR, RALP(CRALP, WPLRA, BPLRA) and Rhizoma Zingiberis (RZ). This study provides scientific basis to some extent for synergy or attenuation of the different compatibility relationship in the clinic application.After compatibility RPA, the change of the contents of total alkaloids in the different medical material and different ratio is very little;However, that content is significantly decreased in artificial gastric juice and artificial intestinal juice. After compatibility RPA, the change of the content of ester alkaloids increases, whether it is in the different medical material and different ratio or in artificial gastric juice and artificial intestinal juice, however, the effect in the artificial gastric or intestinal juice is bigger. After compatibility RPA, the contents of total diester alkaloids is slightly decreased, the decrease in crude material is bigger than prepared materials, with the increase of RPA and has descended tendency; in the direction of medical materials, the stability of MA and HA in RAK is stronger than AC, the stability of AC...