Histone Deacetylase Inhibitor Sodium Phenylbutyrate-induced Human Leukemia Cell Line Kasumi-1 Growth Inhibition, Differentiation And Apoptosis Gene Expression Changes Of Aml1, Aml1-eto Regulate Gene Transcription In Mdk

Objective: To explore the changes of gene express profile of the Kasumi-1 cell line which showed growth arrest, differentiation and apoptosis when treated by histone deacetylase inhibitor, phenylbutyrate (PB); and to determine the role of AMLl and AMLl-ETO fusion protein in MDK gene transcription.Methods: The differentiation and apoptosis of Kasumi-1 cells were analyzed by flow cytometry an (?)nexin V apoptosis detection kit. The gene express profile was detecte HgU95Av2 cDNA microarray and confirmed by semi-quantitative R(?)PCR. The MDK promoter/enhancer (MDKp) sequence with runt domain binding sites was amplified from the human genome DNA and directionally cloned into pGL3-Basic Luciferase reporter vectors to construct the MDKp-pGL3-B expression plasmids. Vectors with mutated runt domain binding sites (mu-MDKp-pGL3-B) were also constructed from the MDKp-pGL3-B by site-directed mutagenesis. Plasmids of the pCMV5-AML1/AML1-ETO and the MDKp-pGL3-B or mu-MDKp-pGL3-B were then co-transduced into 293T cells and the luciferase relative value was analyzed. Results: The expression of the differentiation antigens, CD11b and CD13, were increased when Kasumi-1 cells treated with 3mM PB for 72h; and the early and late apoptosis of the cells were also induced. The...