Across The Blood-brain Barrier

PLGA is a kind of degradable high molecular polymer with good biocompatibility. Drug connected have the function of control release. Our previous research indicate that PLGA with 20000Mw has the best controlled release effect. Adriamycin is added into PLGA to make controlled released drug delivery system with different dose. This part is mainly to investigate the effect of control released system with different drug dose on normal rat brain. Five different dose of control released system (0%, 5%, 10%, 20%, 40%) were buried in normal brain tissue to investigate the effect on it.Methods: Normal Wister rat were devided into six groups. Each group was implanted control released system of certain dose of Adriamycin. After imlpanted, the physiological index was observed, as well as the electron microscope and HE staining. The data was analysed to identify the maximum dose of compatiblity. The effective distance of control released system effect was also investigaed. Results: The the physiological index including life span, exploratory behavior, hanging test, crossing stick test, tiltboard test, blood pressure were observed and statistically analysised. There were no differencial significant between group on life span and the exploratory behavior. There were no differencial significant between groups (control, adjuvant, 5%, 10%) on hanging test . But there were differencial significant in 20% and 40% with the control. There were differencial significant in 20% and 40% with control on balance test, and no differencial significant between groups (control, adjuvant,5%,10%). There were differencial...