Study On The Analgesic And Anti-inflammatory Components Of The Traditional Chinese Medicine "Stauntonia Chinensis" And The Cytotoxic Components Of Mangrove Fungus "Aspergillus Awamori"

Stauntonia chinensis DC. (Lardizabalaceae), an evergreen herb, commonly known as "Ye Mu Gua", has been used as a traditional Chinese medicine, especially for analgesic, sedative and anti-inflammatory purposes.The 60% EtOH extract of the rattan of the plant exhibited analgesic effect, evaluated by the acetic acid twisting method. Farther study showed that the n-BuOH soluble fraction of the 60% EtOH extract was responsible for the analgesic effectBy systematic isolation, the n-BuOH soluble fraction was divided into several sub-fractions. The finger prints were established for the major subtractions. The major components of the major subtractions were isolated by chromatography methods, and their structures were elucidated by spectroscopic analysis and chemical methods. Thus, the major peaks in the finger prints were identified unambiguously. And the minor peaks were deduced by the technology of LC-MS.The compounds from the major subtractions are classified as pentacyclic triterpenoids, isochlorogenic acid analogs, lignanoids, flavonoids. Pentacyclic triterpenoids are the major components, the most aglycones of which are akebonic acid, hederagenin, 30-norhederagenin; and the sugar moieties of which are composed of glucopyranose, rhamnopyranose, arabinopyranose, xylopyranose, galactopyranose, glucopyranuronic acid; and twenty-six pentacyclic triterpenoids of which are new compounds, named Stauntoside A—Stauntoside Z. Isochlorogenic acid analogs are isolated for the first time from Stauntonia chinensis DC.The character of the ESI- MS~n of the isochlorogenic acid analogs and the triterpenoids was investigated, based on which the methods of rapid analysis for the isochlorogenic acid analogs and the triterpenoids were established.The analgesic, sedative and anti-inflammatory effects of the pure compounds are undergoing. Compounds 6-7, 13-13, 18, 20-23, 27-28, 36, 38, 42 exhibited the inhibitory activity on T lymphocyte proliferation derived from Con A, which may be responsible to anti-inflammatory effects of Stauntonia chinensis to some extent Furthermore the isochlorogenic acid analogs exhibited the potent inhibitory activity on the release of the histamine of mast cells. In our searching for bioactive agents from the sea, About 200 fungi strains were collected from mangrove area at Wenchang, Hainan Province. Cytotoxic screening of the extracts of their mycelia presented 8 mangrove fungi exhibiting potent cytotoxic activities.A strain exhibiting potent cytotoxic activity, No. 094811(Aspergillus awamori), was fermented and the acetone extract of the mycelia was isolated. The inhibitory activities on B 16 cell line proliferation of the fractions from the acetone extract of the mycelia of Aspergillus awamori were assayed by MTT method. Several (Give a number) bioactive polyhydroxysterols from the active fractions were isolated by chromatography methods. These known sterols have been reported to exhibit inhibitory activities on cancer cell line proliferation. The inhibitory activities on cancer cell line proliferation of new compounds are undergoing.Up to now, 27 compounds were elucidated by spectroscopic methods. Fifteen compounds were polyhydroxysterols and polyhydroxysterol fatty acid esters, of which all six new compounds were polyhydroxysterol fatty acid esters and the related fatty acids and monoglyceride were also identified. Besides, a series of Cyclic dipeptides and nucleosides were isolated and idenfied.