Series Of New Superparamagnetic Iron Oxide (spio) Magnetic Resonance Contrast Agent Preparation And Application Of Experimental Research

PURPOSES: The goals of the present study were 1. to synthesis a new form of Superparamagnetic iron oxide (SPIO) partical MR contrast agents (SPIO-I and SPIO-2) with narrow size range, which meet intravenous administration requirements;2. to investigate thoroughly the physiochemical properties, pharmacokinetics toxicity and efficacy of SPIO-1 and SPIO-23. to evaluate the potential application of SPIO-1 to improve MR detection of liver carcinoma with MRI in a nude mouse model bearing human hepatocelluar carcinoma (Human 7402 cell line) by subcutaneous implantation (n = 4) and surgical intrahepatic implatation (n = 4).4 to analys the MR features of acute myocardial infarction established by coronary artery occlusion in mongrel dogs (n = 6) and correlate MR findings with histopathologic changes.5. to evaluate the role of SPIO-2 as a blood pool MR contrast a-gent during its intravascular phase in case of myocardial infarction.MATERIALS AND METHODS1. SPIO-1 and SPIO-2 were synthesized directly by reating a mixture of stoichiometrically quantitative ferrous chloride and ferric chloride with dextran polymer under alkaline condition, different sizes were obtained by change reaction temperature, the size distribution of SPIO-1 and SPIO-2 were measured by high resolution transmision electron microscopy. X-ray powder diffration was used to determine the crystal structure in REGAKU-2038 x ray diffrac-tometer. the yielded data was then compared with standards published by JCPDS. the MR signal intensity (SI) of agar phantoms with various concentration of SPIO-1 under varying conditions of...