Electric Adjustment Mechanism Of Action For Chronic Stress Rat Model Of Hpa Axis-glu-nmda Receptor-no Path

Depression is one kind of frequent mental disorder and the lifetime prevalence is up to15 -20%.T'he high disease incidence and harm induced by it have gotten more and more attention in medical science realm.In recently years,many types of anti-depression medications came to the market. However,their effectiveness is limited to controlling certain symptoms. And almost 30% patients with major depression have no reaction to any medicine.Other than the unwanted side-effects such as increased toxin in blood stream, the patients often exhibit behavioral problems such resistance to following orders.An effective therapeutic method is being demanded urgently.Acupuncture,one of the major parts of Traditional Chinese Medicine,has a universal function which can balance the situation of the people. Moreover, it has the prevalence of no side effects and has been acceptted widely in clinical practice. It has been laid more and more focus throughout all over the world.But the research is just on the early period ,and the mechanism of the electroacupucnture (EA) effects depression is still need further exploration.This research emphases on the HPA axis-Glu-NMDA receptor-NO path. We choose the long-time unpredicted chronic stress model that can stimulate the predisposing cause of the depression. we use the frequent most of the acupoints and try to provide basic theory for clinical practice.Analysis the effects of EA on the hormones and related receptors in model rats,we try to explain the mechanism of how EA effects depression.We fetch in MK-801 in our reseach, explore this path from diffirent points and try to explain the mechanism.Discuss if the EA effects NMDA receptor pathway is the mechanism of acupuncture.Methods and conclusion:1.Seven kinds of chronic stress are used unpredictable.Conclusion:the model which we selected is a feasible model,and has been widely used in medical research field.2.We studied the depression level of the model rats by open-field and swim experiment. Evaluate if we made a successful long-time chronic stress model. Analysis EA effects model rats` behavior.Conclusion:⑴By open-field experiment, we observed the rearing times and the crossing times of the model rats. The results show that the rearing times and the crossing times of the rats in the EA group significantly increased compared with the rats of the model group (P<0.01). This results indicate that the EA therapy can improve the activities and the explorative interest of the model rats.⑵We observed the swim of the model rats. The result shows that the stillness time during swim of rats in the EA group decreased significantly compared with the rats of the model group (P<0.05). And the stillness time of the rats in the EA group approximate to that of the MK-801 group. These results indicate that the EA therapy can increase the sensibility of the model rats.3. Analysis the CRF in hypothalamus,ACTH in pituitary gland,CORT in adrenal gland by RIA method.Conclusion:CRF and CORT increased greatly in model group (P<0.05).EA decreased the CRF and CORT level significantly and compared with MK-801group has no difference.4.Analysis the CORT and Glu level in hippocampus by RIA method.Conclusion: CORT and Glu level in hippocampus increased greatly in model group (P<0.05).EA decreased the CORT and Glu level in hippocampus significantly and compared with MK-801 group has no difference.5. Analysis the NMDA receptor level in CA3 immuno histochemical study.Conclusion: the NMDA receptor level in hippocampus increased greatly in model group (P<0.05).EA decreased the NMDA receptor level in hippocampus significantly and compared with MK-801 group has no difference.6. Analysis the NR2A,NR2BmRNA level in hippocampus by Real-time Quantitative ReverseTranscription-polymerase Chain Reaction method.Conclusion:the NR2A,NR2BmRNA level in hippocampus increased greatly in model group (P<0.01). EA decreased NR2A,NR2BmRNA level in hippocampus significantly (P<0.01). And compared with MK-801 group has no difference.7.Analysis the iNOS level by RIA methord, NO3 - level by colorimetry method.Conclusion:the iNOS and NO3– level in hippocampus increased significantly (P<0.01). EA decreased the iNOS and NO3– level in hippocampus significantly (P<0.01). And EA compared with MK-801group has no difference.