| Gingivitis is a common disease with the incidence of more than 90%. It is a disease caused by many factors such as plaque, bacteria and its metabolic productions. As the disease developing, periodontium is destructed, attachment is lost, periodontal pocket goes deepen, tooth becomes mobile and even lost. Therefore, it is the key for periodontal therapy to eliminate inflammations and promote the destroyed gingival tissue regeneration. Nowadays, routine clinical treatment remains as basic periodontal treatment and supplemented with systematically or locally administered antibacterial medicine. But, systematic usage of antibiotics for a long time would lead to unpleasant side effects such as disbacteriosis, drug resistance. The relatively low effective concentration of antibiotics exist in local tissue. The preparation of locally administered periodontal medicine are mouth rinse, films, softcream, paste, glue. The effective components are mainly cetylpyridinium, fetorides and antibiotics. Although locally administered medicine can avoid systematic side effects, the preparation still have shortcomings, such as bad taste and drug resistance. People desire to an ideal preparation wich can not only eliminate inflammations and inhibit bacteria but also promote gingival tissue regeneration.Xipayi mouth rinse wich mainly made of Turkish gall was used to clinic for more than ten years and proved to be very effective drug for periodontal disease including gingivitis, where as its some mechanisims are still unknown. This study give us its scientific bases to be used in clinic widely by①Studying the role of NF-κBp65 and COX-2 in the pathogenesis of gingivitis and the effect of Xipayi mouth rinse on gingivitis at the mRNA level.②Observing the effect of Xipayi mouth rinse on different concentrations on the activities of interleukin-6 (IL-6) from human gingival fibroblast (HGF) induced by lipopolysaccharide (LPS) and the effect of Xipayi mouth rinse on the DNA synthesis and the changes of cell cycle of human gingival fibroblast (HGF) inhibited by lipopolysaccharide (LPS).③Investigating the effects of Xipayi mouth rinse on the control of dental plaque in cases with gingivitis. The study was complete by the means of①Wistar rats were randomly divided into 3 groups: normal group, gingivitis model group and Xipayi mouth rinse group, and experimental gingivitis model was established in the last 2 groups by means of suturing the neck of right upper second molar of rats, high–carbohydrate diet and P.gingivalis inoculation, Wistar rats in Xipayi mouth rinse group treated with Xipayi mouth rinse while other 2 groups treated with 0.9% sodium chloride, the expression of NF-κBp65 and COX-2 mRNA in gingival tissue of 3 groups were examined by RT-PCR.②HGF were stimulated with LPS at the concentration of 25ug/ml, radioimmunoassay (RIA) was used to examine the effect of Xipayi mouth rinse at the concentrations of 12.5-200 ug/ml on the secretion of IL-6 in the supernatant of the cell culture; Flow cytometry was used to examine the effect of Xipayi mouth rinse at the concentration of 50μg/mL on the DNA synthesis and changes of HGF cell cycle.③Patients with gingivitis were randomly divided into therapy group and control group. The Xipayi mouth rinse in therapy group and 0.9% sodium chloride in control group were used by patients for 7days and 5times each day, plaque index(PLI), gingival index(GI), sulcus bleeding index(SBI) and other clinical indexes were measured before and after treatment. Subgingival plaque sample were collected and stained by congo red solution. A total of 200 bacteria were counted including spirochaeta, coccus, bacillus, fusiformis and campylobacter. Compare the clinical and microorganism indexes between two groups.The results of study suggest that①Gingivitis model established succesfully in gingivitis model group, In comparision with the normal group, the mRNA expression of NF-κBp65 and COX-2 increased (P<0.05) in gingivitis model group, while the expression of NF-κBp65 and COX-2 decreased (P<0.01) in Xipayi mouth rinse group.The expression of NF-κBp65 had a relation to COX-2 in gingivitis model group (P<0.05).②IL-6 secreted by HGF was significantly inhibited by Xipayi mouth rinse in a dose-dependent manner, the inhibitory effect of Xipayi mouth rinse at the least concentration is approximately equal to the effect of dexamethasone; The percentage of HGF in G1 phase during exponential growth was increased by LPS, while the percentage of HGF in S phase was decreased as well as proliferation index (P<0.01). Xipayi mouth rinse ameliorated this phenomenon.③PLI, SBI , GI and other clinical indexes in therapy group were improved significantly (P<0.01)after seven- day washing, as well as percentage of spirochaeta, bacillus in dental plaque were decreased and of coccus was increased (P<0.01, P<0.05, P<0.01). In addition, the effectiveness of treatment in experimental group was significantly better than that in control group (P<0.01). So we can conclude that①The expression of NF-κBp65 related to COX-2 may take part in the pathogenesis of gingivitis; Xipayi mouth rinse can inhibit the secretion of pro-inflammatory cell factor IL-6 from HGF induced by LPS. Xipayi mouth rinse may take role in the treatment of gingivitis in this way.②Xipayi mouth rinse can significantly ameliorate the inhibitory effect of LPS on the prolifiration of HGF, thus suggesting the gingival tissue regeneration effect of Xipayi mouth rinse in treatment and prevention of gingivitis.③Xipayi mouth rinse can control dental plaque and relieve gingivitis effectively.④This study give us its scientific bases to be used in clinic widely. |
PDF Full Text Request