| Epilepasy are a kind of common diseases in the nervous system, it is incidence rate for 0.5-2% in the crowd. Currently, although research of epilepsy obtain certain make progress, for etiopathogenisis and pathogenesis of epilepsy currently still is not complete clear. A great deal of of the data manifestation, epilepsy and infalammatory reaction of central nervous system (CNS) relation close, inflammatory reaction come to a decision epilepsy to some extent of occurrence, development. Valid of inhibit inflammatory reaction in brain, result in changing the excitability of the neuron, attain the purpose of the anti-epileptic. But microglia play a critical role in the inflammatory reaction of CNS, activated microglia may secrete a number inflammatory factors, and the expression of CD40 be a key that inflammatory reaction. CD40 is a member of the tumor necrosis factor (TNF) receptor family. It interaction with CD40L which is the cognate ligand of CD40 leads to microglia secretion of cytokine and mediators of inflammation, and mediated inflammatory reaction. However, the CD40 mediated inflammatoory reaction whether also occurenc in epilepticus inflammatory pathology change? Currently, the related research temporary having no this aspect.The acupuncture is an importance in the motherland medical science of non- medicine therapy, it at domestic and international already creation graveness influence. Study displayied, the acupuncture may influence inflammatory reaction by different mechanism, and adjust to release of cytokine and mediators of inflammation. Meanwhile, our study groups previously reported that electric acupuncture (EA) may available inhibited spontaneous recurrent seizure(SRS) through stimulus"baihui"and"dazhui"in the lithium pilocarpine-induced epileptic model. However, this function of EA, whether relation it regulate inflammatory reaction after seizure? This the demand be further study. Moreover, data have displaied that carbamazepine (CBZ) as the anti-epileptic drug (AEDs) have to inhibit inflammatory reaction and neuroprotective function. It not only can inhibit epileptic seizure, but also can influence inflammatory reaction process through different mechanism, alleviate clinical symptom that inflammatory reaction's cause. However, oxcarbazepine as be derivative of carbamazepine, it whether also same have the anti- inflammation function? Whether it possess brain protection function through amelioration inflammatory reaction after seizure? Currently, domestic and international didn't yet the related research of this aspect.Aim at the above-mentioned problem, this topic choose to use the lithium pilocarpine-induced epileptic model, then through immunohistochemistry, Western blot, and ELISA etc, respectively. Firstly, observation microglia change and CD40 expression after status epilepticus (SE) in the experimental epileptic rat. Then, we carry on an intervention to the the epileptic rat through stimulating"bai hui"and"da zhui"two acupuncture points and administration OXC, and observation EA or OXC influence on CD40 mediated inflammation after SE, and two carry on contrast research. Secondly, we have observated EA or OXC intervention to influence of hippocampal neuronal injury after SE in the rat by Nissl staining. End, we further observation EA or OXC intervention to influence of SE and SRS in the rat.Main research's result is as follows:(1) Microglia propagating and activating,and CD40 expression increasing in the different areas of hippocampus after SE. And CD40 expression increase on the activated microglia, but no observation CD40 expression on the astroglia.(2) EA or OXC intervention dissimilarity degree inhibited proliferation and activating of microglia and CD40 expression in the hippocampus of rat, with OXC most is obvious.(3) EA or OXC intervention inhibited TNF-αexpression in the hippocampus of rat, and inhibiting of OXC the function is strong at the EA stimulate.(4) EA or OXC intervention improved neuronal loss of hippocampus after SE, with CA1 area most is obivous, and neuroprotective of OXC is strong at the EA stimulate.(5) EA or OXC intervention not influence development of SE, but they dissimilarity degree extended latency of SRS, and decreased frequency of SRS. In summary, this study demonstrats for the first time that CD40 expression hippocampus in the experimental epileptic rat.This results have showed that CD40-mediated inflammatory reaction occurs in epileptic pathology change. Meanwhile, carrying on EA or OXC intervention may inhibited CD40 mediated inflammatory reaction after SE. Whereafter, our experiment also confirmed EA and OXC possess effects which may protect brain and inhibit SRS.This results showed that EA and OXC may improve neuronal loss of hippocampus through inhibiting CD40 mediated inflammatory reaction, and influence development of SRS, eventually. Therefore, this study not only provided theories foundation and experiment data for EA treatment of epilepsy,but also provided valid basis for studying neuroprotecive effects of AEDs in the"epileptic brain".Our data also provied evidence that"synchondrosis of EA and medicine"for treatment of epilepsy. |
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