Clinical Research Of Traditional Chinese Medicine On Early Metaphase Parkinson's Disease And Empirical Study Of Mscs's Isolated Culture And Depuration Out-of-body

Parkinson's disease(PD)is a kind of degenerative disease of the central nervous system in the middle-age and old age.The disease incidence and sickness rate of PD have the ascending tendency gradually.Sampling survey done by Zhang Zhenxin indicates PD morbidity rate is 1.7%among 29,454 people in cities including Shanghai,Beijing and Xi' an.The morbidity rate is 2.74%in 75~84 age group;4.07%in above 85 age group.With the development of the social, its high incidence and morbidity harmed the human health seriously.The treatment of PD is very difficult.Up to the present,there is not a therapy that could cure PD totally.The PD patients need treatment in their whole life, which brings about enormous loss of social and economy.L-dopa has still been a "the gold standard" of the therapy of PD in that it has advantages of quick effect and easing the symptom obvious etc.This therapy could improve the PD's symptom,however,it would have no command over the PD progress.Moreover, with the extension of the medicine time and the enlargement of dosage,L-dopa's effect would decline gradually and its poisonous side-effects would increase by degrees.Surgical operation has a better effect in the near future,however, its curative effect of long-term is disappointing.Although the research of gene therapy indicates the exciting foreground,the gene transplant is still placed at the experimental stage only and is faraway in the clinical application because of the immunity repulsion,the problem of the ethics and so on.In the recent years,it has been accepted by degrees to treat PD with TCM by more and more people.People believe that TCM therapy could not only improve the PD's symptom but also command over the PD progress.For this reason,to deep understand the clinical therapertie effect of traditional chinese medicine to cure the early metaphase PD.We use RCT and the latest pubished diagnostic criteria therapeutic effect in 2006 to observe the therapeutic effect of traditional Chinese medicine based on the finished clinical research fundation.Meanwhile we progress the testing;trial of rat Mscs isolated culture and depuration out-of-body.PartⅠClinical research of traditional Chinese medicine on early metaphase Parkinson's diseasePurpose:Although the course of PD is long and the treatment is difficult,it provides a very long treatment time for us at the same time.Therefore,we should make use of this good opportunity and develop the TCM treatment of PD, which is one of the direction that we work hard.This research adopts the method of the clinical random parallelism comparison and observes the PD No.1 and No.2 's clinical curative effect and security,trying to look for a new way to the PD.Method:This study elects the PD patients as studied objects to take a prospective random parallelism comparison survey.We gained the random numbers from pems3. 1 for windows which refer to EG:CG=1:1 amounting to 60.We divided the patients into 2 groups simply for the time that they came into our hospital,and gave them PD No.3 and madopar or herb placebo and madopar referring to the randomization.The test ran referring to the test requirement rigorously.At last,we collected 60 cases.All cases of EG and CG has not been lost.Result:According to the comparison of age,gender,family history,Hoehn-Yahr classification standard and the degree of dysfunction of life before treatment, analysis of data indicates that the difference of two group before treatment is not significant,the basal line of two groups is coincident,and with comparability.The outcome shows:Contrast the integral of UPDRS of two groups of prior-treatment to which of post-treatment,the EG's integral of post-treatment of phychosis,behavior,emotion,ordinary activity and movement function has decreased than that of prior-treatment.The difference is significant(P＜0.05).However,the EG's integral of post-treatment of complication is not improved significantly(P＞0.05).The CG's integral of post-treatment of ordinary activity and movement function have decreased than that of prior-treatment.The difference is significant(P＜0.05).However, the CG's integral of post-treatment of phychosis,behavior,emotion and complication is not improved significantly(P＞0.05).Contrast improvement ratio of the integral of UPDRS of two groups of post-treatment,in terms of the improvement ratio of the integral of phychosis,behavior,emotion,ordinary activity and movement function,the EG is superior to the CG.The difference is significant(P＜0.05).But,in terms of the improvement ratio of the integral of the complication,the difference of the two groups is not significant(P＞0.05)。Conclusion:Through the clinical random parallelism comparison trail,we can see there is a better curative effect of the traditional Chinese medicine for patients with PD.When the trail was over,the availability percentage of EG is 86.67 %,while that of CG is为70.00%.The difference is significant(P＜0.05).These indicate that the curative effect of treatment of EG is superior to that of CG.PartⅡempirical study of rat Mscs' s isolated culture and depuration out-of-bodyPurpose:To induce Mscs by means ofβ-ME and to observe the manifestation of nerve cell differentiationMethod:1.The rat Mscs' s isolated culture and depuration out-of-body;2.Use surface molecule signed by FITC or PE to P2 generation Msce in progress flow cytometry identification;3.Nerve cell induced and differentiated by Msce;4.Taking the cells induced after 4h,6h,8h scratch plate and dyied by toluidine blue to observe tigroid body;5,statistical treat datas.Result:The resule demonstrate that induced Mscs appears nerves phaenotype.Nulli-induced Mscs expresses few Nestin and BDNF.Positive Nestin cells notable increase induced byβ-ME.After dyening by toluidine blue,the nerve cell' s cytoplasms have deep blue massive or granulo tigroid body.Conclusion: Through our rsch,we establish a sort of enriching and cleansing Mscs' s method.Which operates simple,easy to form standard.In short-term can get 109 MSCs.Establish the skill platform to science evaluate rat' s Mscs.Use flow cytometry to analyze it' s phaenotype and accredit the purity,direction location experiment can ascertain Mscs' s differentiated potentiality. Utilize the platform,we get a great extent quantitative Mscs with better biological character.