Chemical Constituents And Anti-hepatic Fibrisis Mechanism Of Cassia Mimosoides Linn

Partâ… .The chemical components of Cassia mimosoides linnPurpose:The chemical components of Cassia mimosoides linn were rarely reported,so it is significant to isolate and identify the different chemical components in Cassia mimosoides. Linn.Methods:The fine powder of Cassia mimosoides linn was exacted by 85%ethanol.The extracts were concentrated and the residue was extracted by different solvents with different polarity,in the order of petroleum ether,chloroform,ethyl acetate,n-butanol.The components were isolated and purified by silica gel column chromatography,sephadex column chromatography,reversed phase chromatography,polyamide column chromatography,preparative HPLC.The compounds were identified by physical and chemical properties and modem spectrum methods(UV,IR,MS,¹HNMR,¹³CNMR,DEPT).The data was also confirmed by literature.Results:8 organic compounds were isolated from the ethyl acetate exacts by silica gel chromatography.According to the physical properties and spectral data,they were identified asβ-sitosterol(1),Oleanolic acid(2),Emodin(3),(R)-Artabotriol(4),Daucosterol(5),Phloroglucinol(6),Luteolin(7),α-L-Rhamnose(8). Conclusion:Except for Emodin(3),the other 7 compounds were firstly isolated in Cassia mimosoides linn,and(R)-Artabotriol(4)was firstly found from leguminosae plants.Partâ…¡.The study of cassia mimosoides linn to rats hepatic fibrosisPurpose:To observe the inhibition of dimethylnitrosamine induced hepatic fibrosis and study the mechanism.Methods:The hepatic fibrosis rat modelestabiished by dimethylnitrosamine The detailed process was as follows:Animals were intraperitoneally injected with 0.5%DMN(10mg/kg) solution(saline water/DMN=199/1)at 2/3 of total dosage for successive three days at the first week,and at total dosage at the following 3 weeks.The rats were divided into 8 groups randomly and they are:low,medium,high dose of water-extracted Cassia mimosoides linn groups 10mg/kg(1.4 g/kg,4.2 g/kg,12.6 g/kg,corrsponding to 1,3,9 times of human clinical equivalent dosage),low,medium,high dose of alcohol-extracted Cassia mimosoides linn groups 1 mL/100g(1.4 g/kg,4.2 g/kg,12.6 g/kg),colchicines treated group(0.2 mg/kg)and control group.The rats were fed with drugs by stomach lavaging once everyday for 4 weeks.The rats in control group were fed with saline water(1 mL/100g).At the end of 3rd week,the blood was collected from eye-orbits and the serum was isolated for tests.At the end of 4th week,the rat eyes were extirpated and the blood collected for tests.Then the animals were executed and the left lobes of liver were extirpated and stored in -70℃.The fight lobes of liver were treated with 10%formalin for pathological section tests.According to the user's guide of the reagents,the indexes of rat ALT(alanine transaminase),AST(aspartic acid transaminase),TP(total protein),ALB(globulin),TBA(total bile acid),CHOL(total cholesterol),TG(triglyceride)were determined by automatic biochemical analyzer;The indexes of HA(Haluronic acid),LN(Laminin),PCâ…¢(typeâ…¢procollagen) were determined by radioimmunoassay;The Hyp(Hydroxyproline)of rat liver was determined by basic hydrolysismethod;Light microscope was used to detect the change of liver organs and to grade the hepatic fibrosis. Statistic process:The data was denoted by the standard deviation of average values, and the software SPSS 11.5 was used to do the one-way analysis of variance(ANOVA). The rank data was analyzed with Ridit method.Results:1.The effect of Cassia mimosoides linn to liver function and hepatic fibrosis indexes.When the hepatic fibrosis was completed,the ALT,AST,TP,ALB,GLB,TBA,CHOL,TG,HA,LN,PCâ…¢indexes of control group rats there were statistical difference with normal rats(p＜0.05 or p＜0.01);Compared with the rats in the control group,the ALT levels dropped in the Cassia mimosoides linn dose groups,and the AST,TBA indexes decreased in the high dose alcohol-extracted Cassia mimosoides linn treated groups.The TP,ALB indexes of high,medium dose alcohol-extracted Cassia mimosoides linn treated groups increased apparently.In the water-extracted Cassia mimosoides linn treated groups, the TG index increased in the low dose group(p＜0.05);the HA,LN indexes decreased(p＜0.05).PCâ…¢index decreased in high water-extract dose group and medium,high alcohol-extract dose groups(p＜0.05);Compared with colchicines-treated group,the AST index of high alcohol-extract dose group decreased and the TG index of meidium,high alcohol-extract dose groups decreased(p＜0.05).2.The effect of Cassia mimosoides linn to Hydroxyproline(Hyp)indexes.The Hyp indexes of model rats were higher(P＜0.05)than those of normal rats;The Hyp indexes of high water-extract and all the alcohol-extract dose groups decreased(p＜0.05).3.The effect of Cassia mimosoides linn to rats liver tectology.In the control group,the observation by light microscope revealed that the hepatic lobules structures of model rats were damaged,and the hepatic cords were disordered.The putrescence of some hepatic cells was detected;the vacuolar degeneration of hepatic cells was a decentralized distribution.The stroma hyperplasia was apparent,and the fibrous septa were formed.Pseudo liver lobules were also formed and intact.In all treated-groups, the hepatic lobules structures were ameliorated apparently and there were less fibrous septa. The putrescence of some hepatic cells was rarely detected,and only very few rats had incomplete pseudo liver lobules.In the compare of fibrosis ranks,the hepatic fibrosis indexes in the high water-extract and all the alcohol-extract dose groups decreased(p＜0.05). There is no apparent difference between Cassia mimosoides linn groups and colchicines group(p＞0.05). Conclusions:1.Cassia mimosoides linn can alleviate the inflammation and protect the liver fuction.2.Cassia mimosoides linn can inhibit the hyperplasia of collagenous fibers.In conclusion,Cassia mimosoides linn consists saccharides,anthraquinones,flavones, terpenes,steroid hormones,etc.The extracts of Cassia mimosoides linn can protect the liver fuctions and inhibit the hyperplasia of collagenous fibers.