Phlegm Law In The Bone Marrow Mesenchymal Stem Cell Adipogenic And Osteogenic Differentiation Of Experimental Research,

ObjectiveFrom the point of cytomorphology and marker protein (LPL, ALP, OCN),it observed the effect of adipogenic differentiation and osteoblastic differentiation of BMSCs on Bushen Therapy, Huatan Therapy, Bushen Huatan Therapy, and explore the mechanism of Bushen Huatan Therapy of treating osteoporosis.Method1 .BMSCs of Wsitar rats were isolated and purified by using whole bone marrow adherent method.2.BMSCs was identified by cytomorphology and morphologic characteristics, surface antigens detected by flow cytometer.3.The classic adipocyte-induced system including 1μmol /L dexamethasone, 0.5 mmol/L IBMX, 0.2mmol/L indomethacin and 10μg/mL insulin was used to induce adipogenic differentiation of BMSCs. Through the observsion of cytomorphology ,oil red O staining and the expression of LPL-mRNA , it explored the effect of adipogenic differentiation of BMSCs on Bushen Therapy, Huatan Therapy, Bushen Huatan Therapy.4. The classic osteoblast-induced system including 0.1μmol/L dexamethasone, 10 mmol/Lβ-glycerophosphate, 50μg/mL Vitamin C was used to induce osteoblastic differentiation of BMSCs. Through the detection of alizarin red staining and quantitative, the ALP activity and the expression of OCN-mRNA, it explored the effect of osteoblastic differentiation of BMSCs on Bushen Therapy, Huatan Therapy, Bushen Huatan Therapy.Results1.Using the whole bone marrow adherent method can get good,stable and heterogeneity of BMSCs2. The results of the surface antigens of BMSCs detected by flow cytometer showed the positive expressions of CD29 and CD44 and the negative expressions of CD45. 3.Huatan Herb can make BMSCs reduce the generation of adipocyte, down-regulate the expression of LPL-mRNA.4. Bushen Huatan Herb can promote BMSCs to generate into osteoblast in vitro calci- fication, increase the activity of ALP, down-regulate the expression of OCN-mRNA.Conclusion:The possible mechanism of Bushen Huatan Treatment of osteoporosis is: Huatan Treatment to inhibit adipogenic differentiation of BMSCs, and Bushen Huatan Treatment to promote osteoblastic differentiation of BMSCs.