Development And Research Of PLGA/Type ICollagen/Chitosan Artificial Compound Dura Mater

Objective,to use PLGA,TypeⅠcollagen and chitosan which owns good biocompatibility for the development of new-type compound membrane as an ideal material for dura mater repair.Method:prepare porous PLGA scaffle(P membrane) from 50,000 molecular weight PLGA polymer with solution casting/particulate leaching methods.Then 9:1 and 5:5 Type 1 collagen and chitosan material was compounded onto the porous PLGA scaffle surface to produce PGC(5:5) membrane and PGC(9:1) membrane.100u/cm~2 bFGF was added to PGC(9:1) membrane.Mechanics,waster absorption,factor of porosity, contact angle,scanning electron microscope,cytotoxicity and cell multiplication test were conducted based on the material.PGC(9:1) and bPGC(9:1) membranes with good Mechanics and biocompatibility performance were chosen for further use.Dura mater injury models were designed.The dura mater defects were repaired with PGC(9:1),bPGC(9:1) and autogenous fascia membranes.In 1,2,4,16 weeks after operation,animals were randomly killed in each group and tissues were collected.Visual inspection,HE stain,TEM inspection,Type 1 collagen,bFGF and IL-6 immunohistochemistry tests were conducted.Scar tissue repair,material's adherence to spinal cord tissue, inflammation cell infiltration,Type 1 collagen,bFGF and IL-6 expression profile were observed.Result:PGC membrane and bPGC membrane are superior to PLGA membrane in terms of mechanics and biocompatibility performance.In the animal experiment,non-suture techniques using PGC(9:1) and bPGC(9:1) was successfully applied to repair dura mater defect.Heavier inflammatory reaction was observed in the two compound material groups compared with the autogenous fascia group while the spinal cord adherence was lighter(P＜0.05). Compared with other two materials,PGC membrane inhibited the expression of Type 1 collagen protein,while bPGC(9:1) group had no statistic difference with autogenous fascia group,bFGF in bPGC(9:1) material was found to express in week1 and week2 but not found in week 4.Conclusion:By improving the characteristics of porous PLGA material with Type 1 collagen and chitosan,the mechanics and biocompatibility performance are enhanced.PGC(9:1) and bPGC(9:1) have the best performance among the materials.The inflammatory reaction of the tissue is heavier with PGC material applied than autogenous fascia,although the PGC material can achieve a satisfactory repair of the dura mater.By adding proper amount of bFGF in PGC materials,multiplication and differentiation of fibroblasts are enhanced to improve the repair and replacement of dural tissues.