| Objective:From the theoretical angle,to analyze and summarize the morbility reason of Senile Osteoporosis,to approach the effect of the flavone of rhizome drynaria on the lipidision of marrow stroma cell,as well as the senescence and apoptosis of osteoblast through the experimental study;from the clinical angle,to observe the clinical effect of the flavone of rhizome drynania on Senile Osteoporosis,to supply scientific theory basis for clinical application.Methods:Clinical study:70 patients with Senile Osteoporosis who were depended the diagnostic criteria,were divided into two groups randomly,36 patients in treat group (treated with the Qianggu capsule);34 patients in control group(treated with Caltrate D 600 chew tablet).Appraised the clinical effect of the flavone of rhizome drynania on Senile Osteoporosis,according to the dynamic observation on integrative scores of lumbodorsal pain and lumbodynia,"VAS section grading method" on lumbodynia, "Barthel index rating method" on ADL,detected BMD on anterior-posterior position lumbar2～4 vertebra body and proximate of femur including neck/ward/troch,and determined the index of bone transition including BGP/PINP/ICTP through the radioimmunity method before and after treated half a year,then contrast intragroup and selfgroup.Depending on the standard of curative effect,contrast the difference of curative effect between two groups.experimental study:The effect of flavone of rhizome drynaria's serum in blood on cytoactive of marrow stroma cell of rats from analyse of WST-8.After dexamethasone induced lipidsion of marrow stroma cell,based the co-culture system of cell and marrow stroma cell.we use TUNEL standard detect the marrow stroma cells' apotosis,after intervened of flavone of rhizome drynaria's serum.Osteoblast senescence of rats by we uesd t-BHP induce in vitro,after we used the flavone of rhizome drynaria's serum intervene,Se-nescence associatedβ-galactosidase chemi-staining, observe the cell senescence.To detect the osteoblast apoptosis,after cultured rats' osteoblast in the medium including TNF-a or the the flavone of rhizome drynaria and TNF-a,by fluorescence staining DNA of nucleus,and observing ultrastructure under electron microscope.Results:1.The observation results of 6-points Behavioural Rating Scale(BRS-6) displayed,the lumbodosal pain and lumbodynia with pretherapy SOP patients nearly were three or four degree,ignored impossible.There is several impact to daily living.The lumbodosal pain and lumbodynia lowered to one or tow degree after 3 months treated by the flavone of rhizome drynaria,the aware pain vanished.To illustrate the flavone of rhizome drynaria can extently amend the lumbodosal pain and lumbodynia with SOP,the pain obviously released after 3 months.2.The observation results of "VAS section grading method" displayed,the lumbodynia showed midrange pain while rest/prolonged sitting/walking,and heavy-range pain while to turn the body over/to get up/bend from the waist/tenderness/percussion pain before treated,is especial significance while /to turn the body over/ percussion pain.A piece of patients' lumbodynia impacted their sleep.Various kinds of lumbodynia amended diversity extently through the flavone of rhizome drynaria treated after 3 months.After 1 month,the effect of lumbodynia on sleep is extremely trifling;after 2 months,the majority of rest and prolonged sitting pains nearly vanished;after 3 months,the majority of pains while to turn the body over/to get up/bend from the waist/tenderness/percussion pain can amend,but a piece of patients still showed the pain that bend from the waist and the percussion pain.From that to illustrate the flavone of rhizome drynaria can quickly and obviously amend the lumbodynia that SOP patients showed in static life style,but amend slowly in dynamic life style and surrounding intervention.3.The observation results of "Barthel index rating method" on ADL displayed,the majority of patients' scores were 60～40 before treated,their life with moderate disability and dysfunction need help.After 3 months,the scores can achieve above 90,life nearly self cared,living independence amended extently,quality of life improved quietly.4.The results that detected BMD on anterior-posterior position lumbar2～4 vertebra body and proximate of femur including neck/ward/troch displayed,all positions' BMD improved extently through treated by the flavone of rhizome drynaria after 6 months.The treat group has more conspicuous therapeutic efficacy than control group,there is a significant difference between the two groups,except the BMD on anterior-posterior position lumbar2～4 vertebra body.To illustrate that the flavone of rhizome drynaria can improve proximate of femur bone strength,and prevent fracture of hip. 5.The results that determined bone metabolism maker displayed,the SOP patients' BGP and PINP lower than normal adults,to illustrate that the SOP patients' osteoblast function decayed,synthesis collagen decreased,and bone formation capability weaken.After 6 months treated,BGP and PINP all upgraded extently,to illustrate that the flavone of rhizome drynaria can promote collagen synthesis, improve bone transition,and increase bone formation.The SOP patients' ICTP more advanced than normal adults,to illustrate that the SOP patients' osteolysis increased and bone resorption was more quickly.After 6 months treated,the SOP patients' ICTP lowered extently,to illustrate that the flavone of rhizome drynaria can delay the bone resorption.6.The study results that the effect of flavone rhizome drynaria's serum on cytoactive of marrow stroma cell of rats indicated,the marrow stroma cell's quantity significant increased after intervened of flavone of rhizome drynaria's serum in,to illustrate that the flavone of rhizome drynaria can improve the cytoactive of marrow stroma cell of rats and promote its multiplicative division.7.The study results that the depressant action of flavone rhizome drynaria's serum to osteoblast apoptosis of rats induced by TNF-a indicated,osteoblast cultured in vacant serum adding TNF-a showed apoptotic signs,and the quantity of apoptotic osteoblast significant increased.Osteoblast cultured in flavone rhizome drynaria's serum that its apoptotic significant decreased,although has the chang of apoptotic morphous,also can partially antagnoise osteoblast apoptosis with TNF-a.8.The study results that the protection of flavone rhizome drynaria's serum to apoptosis of lipidsion marrow stroma cell of rats indicated,adipocyte can induce apoptosis of marrow stroma cell,and it significant decreased after intervened of flavone of rhizome drynaria's serum,to illustrate that the flavone of rhizome drynaria can protect the apoptosis of lipidsion marrow stroma cell of rats.9.The study results that the effect of flavone rhizome drynaria's serum on osteoblast senescence of rats indicated,quantity of osteoblast Se-nescence associatedβ-galactosidase which cytochmical staining positive increased obviously after induced by t-BHP,but the quantity of osteoblast senescence decreased obviously,after intervened of flavone of rhizome drynaria's serum,to illustrate that the flavone of rhizome drynaria can protect the osteoblast senescence of rats induced by t-BHP.Conclusions:1.The attack rate of SOP is gradually increasing with the mankind life extending and population aging,seriously jeopardizing the aged health and life.2.SOP is ingredient of the organism natural retrogression and aging process.Its pathogenetic cell pathology is marrow stroma cells' different direction changing and osteoblastics' biological function decaying,as well as,is intimate correlated with five viscera consumptive disease and Qi-blood functional disorder,renal deficiency is the core pathogenesis of that.3.Clinical study indicated:the flavone of rhizome drynaria can promote collagen synthesis,improve bone transition,increase bone formation,delay bone resorption, furthermore,improve SOP patients' integer bone mass and proximate of femur bone strength,and prevent fracture of hip.Eventually,the level of living independence amended and the quality of life improved overall.4.Experimental study indicated:the flavone of rhizome drynaria can improve the cytoactive of marrow stroma cell of rats,promote its multiplicative division,and protect the marrow stroma cells' apotosis with lipidsion.The flavone of rhizome drynaria's serum can extently protect the osteoblast apoptosis of rats induced by TNF-a and the osteoblast senescence of rats induced by t-BHP.The results of empirical study to illustrate that the flavone of rhizome drynaria has considerable significance to improve the bone formation action of SOP. |
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