Experimental Study On The Pharmacological Mechanisms On Cellular Immunity Of BiQingYin For The Treatment Of Rheumatoid Arthritis

Objective: To study epidemiology and pathogenesis of rheumatoid arthritis (RA) in TCM, and Study on the pharmacological mechanisms on cellular immunity of BiQingYin for the treatment of Rheumatoid Arthritis. Scientific evidence of this research would make TCM on treatment on developed further. Methods: As the classical experimental model of T Lymphocytes, Jurkat cell was used to study the pharmacological mechanisms on cellular immunity of BiQingYin for the treatment of Rheumatoid Arthritis. Select TGP, MTX, and DEX as the control group. Investigate the effection of cellular immunity on activation ,differentiation, proliferation, cell cycle and signal molecule of T lymphocytes whish played a important in RA pathological process with immunological methods like flow cytometry, MTT and ELISA.Results: BiQingYin could inhibite the proliferation of Jurkat cell, down-regulated the levels of cytokine IL-2 and IFN-γby activated T cells, down-regulated the expression of CD69 and CD25 expressed on T lymphocytes in peripheral blood mononuclear cells from RA. It showed significant different in BiQingYin group and the model group (p<0.01 or p<0.05) and showed insignificant different (p>0.05) in BiQingYin group and the control group. FACS assay showed that the G1 term was prolonged by high and moderate doses of BiQingYin. Conclusion:The pharmacological mechanisms on cellular immunity of BiQingYin for the treatment of Rheumatoid Arthritis is related to inhibit the activation ,differentiation, proliferation, cell cycle and signal molecule of T lymphocytes...