Shengmai Detoxification Capsules On Micro-inflammatory Intervention On The Experimental Diabetic Rat Myocardium

diabetic cardiomyopathy(DCM)is an important microvascular complication of diabetic mellitus(DM) as well as the common reason resulting in chronic heart failure.Systematic studies on the prevention and treatment for DCM by ShengMaiJieDuTongLuo capsule(SJT capsule) had been performed from the aspects of reference reviews,theory and experimental study in this essay.It layed stress on exploring the relation between the inflammatory pathogenesis of DCM and the pathogenesis theory of the damage of heart by poison and discussing remedial advantages of SJT capsule from intervening inflammatory pathogenesis of DCM."Method of Removing toxicity,removing obstruction and supplementing kiDCMey" being scientific is clarified from different aspects ulteriorly.The experimental study clarifies pathological mechanism of DCM ulteriorly from herb restraining expressed pathway of inflammatory factor,which is practical and innovative.1 Reference review1.1 The skeleton research of traditional Chinese medicine(TCM) on DCMThis essay reviewed from the advances of such fields as followed:the source and name of TCM for DCM,the etiology and pathogenesis for DCM,selection of treatment based on the differential syndrome diagnosis,pathogenesis of TCM on DCM,which is in ancient and modern times,moreover,included the evaluations andprospects on the criterions of diagnosis and curative effect as well as the problems lying in the selection of treatment based on the differential syndrome diagnosis or scientific study.1.2 The latest study of pathogenesis of DCMThis essay summarized the experimental study results and advances on the principle of preventing and treating DCM,according to the pathologenesis of DCM,such as glucoregulation tubulence,fat matabolism tubulence,monocyte chemoattractant protein-1 (MCP-1),angiotensinⅡ(AngⅡ)involving the inflammatory pathogenesis of DCM,et cl.the losing balance of extracellular matrix(ECM),and the relations between them.other etiology, pathogenesis,clinical diagnosis,laboratory examination were also included in recent years years. 2 Theory StudyBy discussing the pathogenesis theory,principle and method of treatment of Xiao ke heart desease with Chinese medicine theories quoting from classics works and combining modern research result,we put forward and perfect the theory that the main mechanism of Xiao ke heart desease is the damnification of heart by poison and we also clarify the relation between the inflammatory pathogenesis of DCM and the pathogenesis theory of the damage of heart by poison.Theory gist of treating Xiao ke heart desease is provided by us from discussing the effective way of "Method of Removing toxicity,removing obstruction and supplementing heart" intervening inflammatory pathogenesis of DCM.3 Experimental study3.1 The influence about SJT capsule on glucose and lipids in blood of diabetic cardiomyopathy ratsSTZ-induced diabetic rats were divided randomly into normal control group,diabetic model group,positive control group(DM+Enalapril),experimental group(DM+SJT capsule of large,median and small dose respectively) in Chinese herb.The various marks were observed.The results showed JDTLBS capsule obviously improve the rats' general condition and had a certain effect on reducing blood sugar,serum cholesterol(TC) and triglyceride(TG), low density lipoprotein(LDL),which had a significant statistic sense in comparing with diabetic model group(P＜0.01,P＜0.05).The results indicated that SJT capsule was beneficial for regulating glucose and lipid metabolism.3.2 Effect of shengmai jiedu tongluo capsule(SJT capsule) on myocardial ultrastructure in Diabetic RatsThe changes of myocardial tissue morphology in experimental diabetic rats were observed by optical microscope and electric microscope.What were seen in model group by optical microscope were that the number of myocardiac interstitial fibrocyte increased, protein substances with positive marked by PAS,Under the transmission electric microscope, the myofilament of myocardial cell disarranged,the number of mitochondrion increased and was agglomerate,the content of the collagenous fiber in myocardiac interstitium increased, Myocardium hypertrophy,a focal loss,disorganization of myofibril,mitochondril swelling, and lysis of cistae were observed。The pathological changes of experimental group and positive control group treated by SJT capsule and Enalapri for 12 weeks were alleviated more obviously than diabetic group.The results showed SJT Capsule may decrease the myocardial injury of diabetic rats significantly.3.3 The study about SJT capsule to decrease of content of AngⅡand decomposite the experimental diabetic rat's myocardial ECMStudying the content of AngⅡin the experimental animals' serum and local myocardial tissue with radioimmunoassay,the results showed:the content of AngⅡin DM modal group' s serum and local myocardial tissue obviously increased,which have remarkable difference from the normal control group(P＜0.01);the content of AngⅡin the serum and local myocardial tissue of experimental group that cured by SJT capsule and postive control group that cured by Enalapril obviously decreased,which have remarkable difference from the DM model group(P＜0.01);So SJT capsule can decrease the content of AngⅡin the serum and local myocardial tissue.Studying the protein expression of ColⅠ,Ⅲin the experimental animals' myocardial tissue with immunohistochemical method and observing the effect of SJT capsule on the protein expression of ColⅠ,Ⅲin the experimental animal's myocardial tissue are to investigate the molecular mechanism of SJT capsule preventing and treating DCM.The results showed:the protein expression of ColⅠ,Ⅲin the modal group's myocardial tissue was in high level,immunostaining of it was increased from intention and area which was more 8 times than that in normal control group.The number of ColⅠ,Ⅲpositive cells in the myocardial tissue of experimental group that cured by SJT capsule and postive control group that cured by Enalapril is remarkably fewer than that in the DM modal group(P＜0.01,P＜0.01).So SJT capsule can inhibit the protein expression of ColⅠ,Ⅲin the experimental animals' myocardial tissue markedly.It indicate that the decrease of content of AngⅡis one of the function mechanisms that SJT capsule prevent and treat DCM though promote the decomposition of ECM in myocardial tissue,which maybe partly had correlation with its ability to interdict the expressed way of inflammatory factors and then have a regular effect on inhibiting the inflammatory pathogenesis of DCM.3.4 The study about effect of SJT capsule on the protein and gene expression pathway of MCP-1 in experimental diabetic rat's myocardial tissueIt was done that assaying the protein expression of MCP-1 in experimental rats' myocardial tissue of each group by immunohistochemical method and the expressive content of MCP-1 mRNA in experimental rats' myocardial tissue of each group by RT-PCR method and observing SJT capsule's effect on MCP-1 in diabetic rats' myocardial tissue,so that we could probe the mechanism of and progress of diabetic glomerular sclerosis.The action of the three factors on the pathogenesis of DCM maybe have concerned with the following:AngⅡcan stimulate the expression of induce the expression of MCP-1 and consequently MCP-1 activate the ColⅠ,Ⅲ,,at last ECM accumulate,DCM is worse.The results showed:the protein expression of MCP-1 and the level of MCP-1 mRNA in modal group obviously increased more than normal control group(P＜0.01);the protein expression of MCP-1 in the myocardial tissue of experimental group that cured by SJT capsule and postive control group that cured by Enalapril obviously decreased,which have remarkable difference from the DM model group(P＜0.01,P＜0.05);It indicated that SJT capsule could obviously inhibit the excess expression of MCP-1 in diabetic rats' myocardial tissue,which maybe partly had correlation with its ability to prevent and treat DCM by inhibiting the way of MCP-1 and intervening the inflammatory pathogenesis of DCM.