Study On Expression Of Breast Cancer Related Proteins And Their Prognostic Significance

Objective:To study the relationships between clinical and pathological parameters and prognosis retrospectively in 405 patients with breast cancer.Analyze the correlations between related proteins' expression and patients' prognosis.Study the molecular classification in breast cancer patients.Methods:Clinical and pathological parameters of breast cancer patients who had been operated in the Cancer Hospital,Chinese Academy of Medical Sciences in the 2000 were collected.Tissue microarrays were established with wax blocked tissue.The expressions of PTEN,Ki-67,Bcl-2,BRCA-1 and CK5/6 proteins were measured with immunohistochemistry technique.The relationships between the expression of clinical and pathological parameters and prognosis were analyzed.Results:All of 405 patients received operation and 70 of them died.Among patients with metastasis disease,most of them were bone and lung metastasis.The survival period were 4～94 months with the median of 71 months.Univariate analysis suggests that clinical staging,expressions of ER,PR,Her-2,p53,PTEN,Ki-67,Bcl-2 proteins,and molecular typing were significantly correlated with patients' over-all survivals(OS),and could be regarded as predictors to evaluate the prognosis of breast cancer patients.Clinical staging, expressions of ER,PR,Her-2,p53,Ki-67,Bcl-2 and CK5/6 proteins were significantly correlated with patients' disease-free survivals(DFS) and could be regarded as predictors of recurrence and metastasis in breast cancer patients.Cox multiple factors survival analysis suggests that PTEN,Ki-67,Bcl-2,CK5/6 proteins expression and staging are independent prognosis factors which are correlated with OS.Staging,Ki-67 and PR status were independent prognosis factors which are correlated with DFS.Conclusion:Molecular classification contributes to the prognosis of breast cancer patients,and could be regarded as one of the factors for evaluating survival time.PTEN, Ki-67,Bcl-2 and CK5/6 proteins expression and staging are independent prognosis factors which are correlated with OS.Staging,Ki-67 and PR status were independent prognosis factors which are correlated with DFS.