Study On The CD4~+CD25~+ Regulatory T Cell In Patient's Peripheral Blood With Psoriasis And Its Role In Psoriasis Immunology

Partâ… The expression of CD4⁺CD25⁺regulatory T cells in peripheral blood of patients with psoriasisObjective:To study the level of regulatory T cells labeled by different antibody in peripheral blood of patients with psoriasis and its role in the pathogenesis.Methods:Flow cytometric analysis was employed to study the CD4⁺CD25^highFoxp3⁺regulatory T cells in the peripheral blood of 45 patients with psoriasis.The expression of CD4⁺CD25^highcells in 45 patients with psoriasis and the surface antigens(CD4,CD25,CD127) of T cells in the peripheral blood of 10 psoriasis vulgaris were also detected by Flow cytometric analysis.Results: The proportion of CD4⁺CD25^high,CD4⁺CD25^highFoxp3⁺regulatory T cells were both significant higher in patients with psoriatic erythroderma than healthy control and other types(P＜0.01,P＜0.05);In psoriasis vulgaris,the CD4⁺CD25^highFoxp3⁺regulatory T cells population demonstrated elevated ratio in guttate psoriasis group,compared to chronic plaque psoriasis;Same change happened to pustule remained pustular psoriasis patients versus pustule faded pustular psoriasis.There was big difference of the percentage of CD4⁺CD25^hi-int CD127^low/- regulatory T cells compared to CD4⁺CD25^highregulatory T cells in both psoriasis and normal,while the psoriatic group has the same level of CD4⁺CD25^hi-intCD127^low/-regulatory T cells as normal one.Conclusion:CD4⁺CD25^high,CD4⁺CD25^highFoxp3⁺regulatory T cells may play an important role in the special pathogenesis of erythrodermic psoriasis;Less number of CD4⁺CD25^high Foxp3⁺ regulatory T cells may not work properly in early stage of the different psoriasis,leading the disease progression.Treg cells can be purified better by CD127 to further study its number in peripheral blood.Partâ…¡The function change of CD4⁺CD25^highregulatory T cell in peripheral blood of patients with psoriasisObjective:To analysis the function change of CD4⁺CD25^highregulatory T cells in patients with psoriasis vulgaris compared to normal group.Methods:Using the immunomagnetic beads isolate the CD4⁺CD25^highTreg cells and CD4⁺CD25^-Tresp cells(T effector).Sixteen hours before finish culture,[3^H]-thymidine was added to analyze alloantigen-specific T cell proliferation of CD4⁺CD25^-Tresp cell in the presence or the absence of Treg cells.The secretive function of Tresp cells,Treg cells co-cultured and cultured separately were measured by ELISA.Foxp3 mRNA level of Treg and Teff cells were also detected by real-time quantitative RT-PCR.Results:The proliferation ability of the CD4⁺CD25^high Tregs and CD4⁺CD25^-Tresp cells in control and psoriatic group have no difference. The CD4⁺CD25^highTreg cell population demonstrated significant decreased suppression to effector T cells(P＜0.01).As secretive function,the level of IFN-γin Tresp cell of psoriasis were much higher than normal group,the suppression of Treg cells to co-cultured IFN-γsecreting of Tresp cells in psoriasis were decreased compared to normal(P＜0.05);While there was no change in IL-10 and TGF-βsecretion level of Treg cells co-cultured in two group.In both psoriatic and normal patient,Treg population contain high level Foxp3 mRNA compared to Tresp.Conclusion:Dysfunction of CD4⁺CD25^highTreg cell may be a potential explanation for unrestrained effector T cell proliferation in psoriasis and play an important role in the pathogenesis of psoriasis.Partâ…¢Extracellular adenosine generation in psoriatic patient' s peripheral blood CD4⁺CD25⁺regulatory T cellObjective:To study CD4⁺CD25⁺Treg cell surface ectoenzyme CD39/CD73 and adenosine receptor A2A expression level on CD4⁺CD25⁺Treg cell and CD4⁺CD25^-Teff.Methods:Flow cytometric analysis was employed to study the CD39,CD73 level on CD4⁺CD25^highTreg cell and CD4⁺CD25^highFoxp3⁺Treg cell in peripheral blood of psoriasis vulgaris and normal patients.The expression of A2A receptor in Treg cell and Teff cell was also detected by Flow cytometric analysis in psoriatic and normal patient.Results:The double positive of CD39/CD73 and single CD73 positive expression in CD4⁺CD25^highregulatory T cell were both significant lower in patients with vulgaris psoriasis,compared to normal control,2.41±0.42vs6.11±1.27(P＜0.01) and 6.48±1.53vs14.28±2.46 respectively(P＜0.01), while in CD4⁺CD25^mde and CD4⁺CD25^-T cell,the psoriatic single CD73 level also decreased(14.34±1.63vs23.34±3.85,15.42±4.46vs28.58±6.42,P＜0.05);After Foxp3 staining,the CD4⁺CD25^highFoxp3⁺regulatory T cell population in psoriasis vulgaris patients still demonstrated obvious decrease ratio in double CD39/CD73 and single CD73 level compared to normal patient(2.89±0.55vs5.97±1.09,7.69±1.49vs12.65±1.98,P＜0.01):No difference of CD39,CD73 level in CD4⁺CD25^med and CD4⁺CD25^-T cell part after Foxp3 staining.The A2A receptor level in CD4⁺CD25^highFoxp3⁺regulatory T cells was significant higher in patients with psoriasis vulgaris than healthy controls,43.7±9.55 and 70.4±7.86 respectively(P＜0.01);No expression difference of A2AR on CD4⁺CD25^-Teff cell in psoriatic patient and normal one.Conclusion:Extracelluar adenosine generated by Treg cell marker CD73 and adenosine receptor A2A may impart the attenuated suppression capacity of peripheral blood Treg cells in psoriatic patient and play an important role in the pathogenesis of psoriasis.