The. Tangzhiping Fat Induced Insulin Resistance In Rat Fat Factor And Protein Kinase B

There are two parts in this thesis:Reference review of insulin resistance (IR),and experimental study on the prevention and treatment for IR by Tangzhiping.1 reference reviewThis essay reviewed from the advances of such fields as followed:reference review of Chinese medicine and modern medicine research.We had a systematic discussion from the pathogenesis theory,syndrome differentiation,and clinical and experimental study for IR with Chinese medicine.At the sametime,we reviewed the pathogenesis and progressof drugs for IR in modern medicine systematically.2 experimental studyObjectivesBased on the prevention and treatment for IGT,and improving IR with Tangzhiping, we investigated the influence of Tangzhiping on the gene expression of adiposederived cell factor and insulin signaling pathway in T2DM and IR,in order to supply the basic theory and experimental data for researching the mechanism of action of the function of the adiposity in IR and T2DM and for the mechanism of action of Tangzhiping.MethodsWeused IR rats model by high fat,and divided them randomly into four groups: normal group,model group,rosiglitazone group,TZP groups.Every group included 10 rats;fat forage induced insulin rat were used as animal models.Model group and normal group were intragastric administrated with sterile water;western medicine group were intragastric administrated with rosiglitazone,and TZP groups were intragastric administrated with TZP separately.After treated correspondingly separately for 8 weeks,general condition were observed in all groups, hyper insulin mice uglycemic clamp were used for evaluate IR.All mice werekilled to collect the blood,adipocyte and the muscle.Adiponectin,TNF-α,leptin, PKB/Akt was detected by RT-PCR.Results1.Rats model induced by high fat had elevatory body weight,blood sugar and fasting insulin,and had a characteristics of lipid metabolism disorder,the descending of glucose metabolic rate.So this model is comparatively ideal IR animal model.2.TZP and rosiglitazone could increased glucose infusion rate(M)(P＜0.05,P＜0.01),and could decreased fasting blood glucose and serum insulin,TC,LDL-C (P＜0.05),decreased the body weight of IR model rats. 3.Rats model induced by high fat had elevatory adiponcetin mRNA levels in the epididymis(P＜0.05,P＜0.01),which ramarkable reduce than normal groups. TZP and rosiglitazone could increased adiponcetin mRNA levels in the epididymis (P＜0.05,P＜0.01).4.Rats model induced by high fat had descending TNF mRNA levels in the epididymis(P＜0.05,P＜0.01),which ramarkable reduce than normal groups.TZP and rosiglitazone could decreased TNF mRNA levels in the epididymis(P＜0.05,P＜0.01).5.Rats model induced by high fat had elevatory PKB mRNA levels in the epididymis and skeletal muscle(P＜0.05,P＜0.01),which ramarkable reduce than normal groups.TZP and rosiglitazone could increased leptin mRNA levels in the epididymis and skeletal muscle(P＜0.05,P＜0.01).conclusionTZP could descended glucose infusion rate(M),improving IR;descended the body weight and improved lipid metabolism disorder of IR model rats;increased adiponcetin mRNA levels in the epididymis;decreased TNF mRNA and levels in the epididymis;increased leptin mRNA levels in the epididymis and skeletal muscle. So TZP have a role of improving IR through effecting the expression of adiponcetin,TNF,liptin and PKB.