The Regulating Effect And Mechanism Of Liver-regulating Compound Recipe On Neurogenesis In DG Region On The Depressed Rats That Stimulated By Chronic Stress

Objective:Currently,we treat the depression more depend on biosynthesis preparation, while some of the drugs has the shortage of addiction and some has the shortage of side affect that has been abused in the clinic.Research proves that chronic stress related to depression.Neurogenesis happening in the dentate gyrus(DG) region is important for maintaining the structure and function of hippocampus.Harmful chronic stress can depress the neurogenesis,and reduce the number of neurons.There has no glossaries in TCM for stress,but the Theory of zang-manifestations and emotion disorder is similar with modern stress theory.Liver-regulating compound recipe is based on the theory of zang-manifestations,and has been used in the clinic widely and effectively for treating the Qingzhi disease.Liver-regulating compound recipe have the whole regulation function, which overcomes some of the shortage of biosynthesis preparation and shows the prospect of development.In this study,we try to find out the effect of liver-regulating compound recipe on depression that stimulated by chronic stress and try to find out some of the mechanisms that behind it in view of imbalance of proliferation and apoptosis happened under chronic stress.Methods:we copy the model of chronic mild stress using Wister Rat in our research, and treating the rats by XYS and JWSNS along with the copying.Get the frozen sections of the brain in the range of target and detect the expressions of the indexes that can be used to detect the effect and explain what we see by insituhybridization and fluorescence Immunohistochemsitry.1,Copy and validate the chronic mild stress(CMS) model;2,Observe the result of Sucrose consumption and Forced swimming;3,Take the frozen sections of rat brains to detect the expression levels of NeuN,BrdU in DG region by fluorescent-labeled Immunohistochemistry;detect the expression levels of apoptosis in DG region by Fluorescein FragEL^TM DNA Fragmentation Detection Kit;detect the expression levels of BDNF in DG region by fluorescent-labeled Immunohistochemistry; detect the expression levels of BDNF mRNA by insituhybridization;detect the expression levels of pCREB in DG region by fluorescent-labeled Immunohistochemistry;detect the expression levels of NMDA R1 in DG region by fluorescent-labeled Immunohistochemistry.Result:1,The average forced swimming immobility time of liver-regulating compound recipe group is obviously shorter than the model group(liver-regulating compound recipe group1 P <0.01,liver-regulating compound recipe group 2 P <0.05).2,The average intensity of fluorescence of NeuN in liver-regulating compound recipe group is obviously higher than the model group(P <0.05) and western medicine group(P <0.01).3,The average intensity of fluorescence of BrdU in liver-regulating compound recipe group is obviously higher than the model group(P <0.01).4,The ratio of the average intensity of fluorescence marking by apoptosis and the average intensity of DAPI which marking all the cells in liver-regulating compound recipe group is obviously lower than that of the model group(P <0.01).5,The average intensity of fluorescence marking by BDNF in liver-regulating compound recipe group is obviously higher than the model group(P <0.01).6,The average intensity of BDNF mRNA in liver-regulating compound recipe group is obviously higher than the model group(P <0.01).7,The average intensity of fluorescence marking by pCREB in liver-regulating compound recipe group is obviously higher than the model group(P <0.01).8,The average intensity of fluorescence of NMDAR1 in liver-regulating compound recipe group is obviously lower than the model group(P <0.01).Conclusion:In our study,we make three conclusions.First,the chronic stress can decreae the proliferation of the neurons,increse the apoptosis of the neurons and decrease the number of the neurons in DG region,which means the chronic stress inhibits the neurogenesis in DG gegion.Second,the liver-regulating compound recipe can accelerate the proliferation of the neurons,depress the apoptosis of the neurons,and increase the number of neurons in DG region to play an important role in anti-depression. Third,the liver-regulating compound recipe can also up-regulate the expression of pCREB, BDNF mRNA,and BDNF in DG region under chronic mild stress,down-regulate the expression of NMDA R1 in DG region,which plays an important role in regulating the neurogenesis in DG region.