Studies Of The Constituents Of Cynanchum Wallichi Wight And Pfaffia Glomerate

Cynanchum wallichii Weight,Duanjieshen,is a traditional Chinese medicine distributed extensively over southwest China.It is used as the primary drug in the famous Chinese prescription "hulisan",which is used to treat arthrophlogosis and injury from fall or fracture.In our investigation,21 compounds were isolated from the 95%ethanol extract of C. wallichii Weight,18 of which were identified as wallicoside B(1),wallicoside C(2), wallicoside D(3),wallicoside E(4),wallicoside F(5),wallicoside G(6),wallicoside H(7), wallicoside I(8),wallicoside J(9),wallicoside K(10),wallicoside L(11),wallicoside M(12), caudatin(13),gagamine(14),otophylloside A(15),otophylloside B(16),cynanauriculoside A(17),otophylloside M(18)。Among them,compounds 1-12 were new compounds, compounds 15-18 were isolated from Cynanchum wallichii Weight for the first time.The growth inhibitory activities of some C₂1 steroidal glycosides in vitro against cancer cell HL-60 and PC3 were measured.Compounds 4,6,13 and 16 showed certain activities against the cancer cell HL-60,and compounds 6 and 16 showed certain activities against the cancer cell PC3.Pfaffia glomerate belongs to the family of Amaranthceace,genus of Pfaffia Pedersen. Pharmacological experiments show that the extracts from Pfaffia glomerate have the effects of enhancing male sexual capacity,anti-inflammatory,analgesic,sedative and anti-diabetes. 41 compounds were isolated from the 95%ethanol extract of P.glomerate,37 of which were identified as:glomerate acid B(1),glomerate acid C(2),glomerate acid D(3),glomerate acid E(4),glomoside A-M(5-17),20-hydroxyecodysone(18),pterosterone(19),taxisterone (20),podecdysone C(21),dihydrorubrosterone(22),calenduloside E(23),6'-ο-methyl calenduloside E(24),chikusetsusaponinâ…£a methyl ester(25),chikusetsusaponinâ…£a(26), boussingoside A₂(27),pfaffoside B(28),oleanolic acid(29),20(29)en-30-noroleanolic acid (30),β-glucopyranosyl oleanolate(31),mesenbryanthemoidgenic acid(32),serratagenic acid (33),22-Oxo-20-hydroxyecdysone(34),11-oxo-β-glucopyranosyl oleanolate(35),22βhydroxyl-oleanolic acid(36),20α-hydroxyl-29-noroleanolic acid(37).Among them, compounds 1-10 were new compounds,compounds 11-17 were new ester compounds.