Expression Of Plasmacytoid Dendritic Cells, TLR9,IRF-7, IFN-αmRNA In The Lesions And Peripheral Blood In Psoriasis

Part I Recruitment of Plasmacytoid Dendritic Cells in the Lesions of PsoriasisObjective To investigate the recruitment and significance of Plasmacytoid Dendritic Cell (pDC) in skin lesions of patients with psoriasis.Methods Twenty-two patients with psoriasis were collected from the clinic and ward of the department of dermatology of our hospital. The diagnosis was based on clinical criteria as well as on histological assessment of formalin-fixed and paraffin wax-embedded samples.The Fifteen healthy controls were chosen from the cosmetic surgery of our hospital. Biopsies were taken from the skin lesions of trunk in patients with psoriasis and corresponding parts of the controls. From each specimen, a portion was snap-frozen in liquid nitrogen and then stored at -70℃until use. The recruiment of Plasmacytoid Dendritic Cells in the lesional skin of psoriasis were detected by immunohistochemical technique(SP), As control, normal skin of healthy volunteers were also tested.Results The immunohistochemistry study showed that CD123+ Plasmacytoid Dendritic Cells were present in all of the patients with psoriasis vulgaris, and the expression outcome is 10±2.1. Few CD123+ cells were observed in controls. the expression of pDC in the psoriatic lesions was significantly higher than that in the normal controls.Conclusion These findings indicate that up-regulated expression of PDC might be related to the pathogenesis of psoriasis. Part II The proportion of Plasmacytoid Dendritic Cells In the peripheral blood in PsoriasisObjective To investigate the proportion and significance of Plasmacytoid Dendritic Cell in the peripheral blood in psoriasis.Methods The proportion of Plasmacytoid Dendritic Cells in the peripheral blood in psoriasis were detected by flow Cytometry According to their CD123+BDCA-2+ phenotype. As control, normal peripheral blood of healthy volunteers were also tested.Results According to the results of flow Cytometry, we found a decrease of CD123+ BDCA-2+pDCs in the peripheral blood in psoriasis patients (0.17±0.07)% compared with the peripheral blood of healthy individuals(0.33±0.20)%, there were significant differences between psoriasis group and control group(P<0.01).Conclusion The finding indicate that proportion of PDC might be related to the pathogenesis of psoriasis. PartIII Expression of TLR9 and its mRNA in lesions of psoriasisObjective To investigate the expression of toll-like receptor 9(TLR9) and its mRNA in the lesions of psoriasis vulgaris.Methods Twenty-two patients with psoriasis were collected from the clinic and ward of the department of dermatology of our hospital. The diagnosis was based on clinical criteria as well as on histological assessment of formalin-fixed and paraffin wax-embedded samples.The Fifteen healthy controls were chosen from the cosmetic surgery of our hospital. Biopsies were taken from the skin lesions of trunk in patients with psoriasis and corresponding parts of the controls. From each specimen, a portion was snap-frozen in liquid nitrogen and then stored at -70℃until use. The expressions of TLR9 and its mRNA in the lesional skin of psoriasis vulgaris were detected by immunohistochemical technique(SP) and RT-PCR. As control, normal skin of healthy volunteers were also tested.Results The immunohistochemistry study showed that the expression of TLR9 in the psoriatic lesions was significantly higher than that in the normal controls. According to the results of RT-PCR,the expression of TLR9 were presented in all specimens of both psoriatic lesions and normal controls. The expression level of TLR9mRNA were 1.2818±0.0902 in skin lesions, which were greatly higher than that in normal controls(0.8681±0.0302)(P<0.01).Conclusion These findings indicate that up-regulated expression of TLR9 and its mRNA might be related to the pathogenesis of psoriasis. PartIV Expression of IRF-7,IFN-αmRNA in the Lesions of PsoriasisObjective To investigate the expression of interferon regulate factor-7(IRF-7) and interferonalpha(IFN-α)mRNA in skin lesions of patients with psoriasis vulgaris.Methods Twenty-two patients with psoriasis were collected from the clinic and ward of the department of dermatology of our hospital. The diagnosis was based on clinical criteria as well as on histological assessment of formalin-fixed and paraffin wax-embedded samples.The Fifteen healthy controls were chosen from the cosmetic surgery of our hospital. Biopsies were taken from the skin lesions of trunk in patients with psoriasis and corresponding parts of the controls. each specimen was snap-frozen in liquid nitrogen and then stored at -70℃until use. The expressions of IRF-7,IFN-αmRNA in the lesional skin of psoriasis vulgaris were detected by RT-PCR. As control, normal skin of healthy volunteers were also tested.Results According to the results of RT-PCR,The mRNA expression of IRF-7 were greatly higher than that in normal controls, but no difference for IFN-α.Conclusion These findings indicate that up-regulated expression IRF-7mRNA might be related to the pathogenesis of psoriasis.