Study On The Constituents Of Artemisia Capillaries Thunb And Morus Alba L Leaves, Liver Cell Membrane Immobilized Chromatography And Its Application For The Active Constituents Of Yinchenhaotang

The water extracts from Artemisia capillaries Thurb and Morus alba L leaves were individually separated by modem chromatographic methods,such as silica gel col umn chromatography,Sephadex LH-20,TLC and semi-preparative reversed phase HPL C,and so on.The structures of compounds were elucidated on the basis of spectrosco pic methods(UV,IR,NMR,MS and CD) and their physical-chemical properties.30 compounds were isolated from A.capillaries Thurb and were identified as 6'-O -caffeoyl-p-hydroxy-acetophenone-4-O-β-D-glucopyranoside(1);9-[1-(3,4- dihydroxyphe nyl)ethyl]benzene-adenine(2);3(R)-deca-4,6,8-triyne-l,3-diol(3);3(R)-decene-4,6-diyne-1, 3,8-triol(4);3(R)-9-deca-4,6,8-triyne-1,3-diol-1-O-β-D-glucopyranoside(5);7-hydroxycou marin(6);6,7-dimethoxycoumarin(7);6-methoxy-7-hydroxycoumarin(8);7-methoxycouma rin(9);4-hydroxyacetophenone(10);4-hydroxy-3-methoxyacetophenone(11);nicotinic aci d(12);benzenecarbinol-O-β-D- glucopyranoside(13);benzoic acid(14);oxalic acid(15); amber acid(16):quercetin(17);hyperoside(18);thymine(19);thymine ribonucleosi de(20);uracil(21);vanillin(22);vanillic acid(23);4-hydroxyacetophenone -4-O-β-D-glucopyranoside(24);2-hydro- benzoic acid(25);3-methoxy-caffeic acid a-4 -O-β-D-glucopyranoside(26);quercetin-7-β-D-glucopyranoside(27);isorhamnetin-3-O -β-D-glueopyranoside(28);loliolide(29);phthalic acid bis-(2-ethylhexyl) ester(30)。25 compounds were isolated from M.alba leaves and were identified as acetyl-lol iolide(1);4-hydroxy- benzoic acid(2);dehydrololiolide(3);loliolide(4);o-dihydrox ybenzene(5):scopoletine(6);benzenecarbinol-O-β-D-glucopyranoside(7);3β-hydroxy-5α,6α-epoxy-7-megastimen-9-one(8);6,7-dihydroxycoumarin(9);cyclo-(Leu-Ile) (10);cyclo(Phe-Tyr)(11);3,4-dihydroxy-benzoic acid(12);isolarieiresinol(13);ci wujiatone(14);7,8-dihydroxycoumarin(15);cyclo(ala-leu)(16);vanillic acid-4-O-β-D-glucopyranoside(17);thymine(18);thymine ribonucleoside(19);quercetin(20); amber acid(21);aspartate(22);thenylalanine(23);valine(24);tyrosine(25)。Bio affinity chromagraphy was a method which use some selective and specific pairs of substance existed in biological environment,such as enzyme-substrate,enzyme -inhibitor,hormone-receptor,antibody-antigen,medicine-receptor to analyze bio active s ubstance.Certain selective analysis and separation were archived by using these pairs. We established liver cell membrane solid phase chromatography in our reseach and pu t it into use in the analysis of active substance in Inchenhaotang.In result,we get 4 selected peaks in Artemisia capillaries Thurh and three of them were identified as 7-h ydroxycoumarin,7-methoxycoumarin and Capillartemisin A.2 peaks were obtained fro m Gardenia jasminoides Ellis and one of the them was identified as geniposide.