| OBJECTIVES1. The aim of this study is to compare the expression level of microRNA let-7a-1,let-7b,let-7c in fresh cervical invasive squamous cell carcimoma tissues(CISCC) to the matched normal cervical tissues(MNCT).2. To compare the expression level of microRNA miR-127-5p,miR-218 in paraffin embedded cervical invasive squamous cell carcimoma tissues to the matched normal cervical tissues.3. To elucidate corelation of miR-127-5p and miR-218 with neoadjuvant chemotherapy, FIGO stage, tumor size, pathological type, differentiation, deep muscular invasion, lymph node metastasis, vascular invasion, and recurrence in cervical caner.METHODSIn this study, microRNA let-7a-1,let-7b and let-7c were quantified in 9 fresh frozen specimens of cervical squamous carcinoma by real-time quantitative PCR array methods. 42 paraffin-embedded cervical squamous carcinoma specimens and their clinical data were collected. microRNA miR-127-5p and miR-218 were quantified in those specimens by real-time quantitative PCR array methods. Correlation of relative expression level of those two microRNAs and several factors were analyzed by SPSS statistical software.RESULTS1. The mean expression of let-7a-1 in CISCC and MNCT was 0. 8828±0. 02075 and 0. 9195±0. 02104; the mean expression of let-7b in these two groups was 0. 8422±0. 03928 and 0. 8891±0. 01052; the mean expression of let-7c in these two groups was0.9467±0.05266 and 0. 9810±0. 07344. Expression of the 3 genes was no significant difference between two groups (P>0.05).2. The mean expression of miR-127-5p in ISCC and MNCT was 1.4931±0.03744 and 1.3662±0.05681; the mean expression of miR-218 in these two group was 1.3403±0.01891 and 1.3114±0.05458. Expression of these two genes was no significant difference between two groups (P>0.05).3. Expression of miR-127-5p and miR-218 in neoadjuvant chemotherapy group and non-neoadjuvant chemotherapy group, FIGO stage I group and stage II group, large tumor size group and small tumor size group, poor differentiated group and well differentiated group, keratinized group and non keratinized group, deep muscular invasion group and non-deep muscular invasion group, recurrent group and non-recurrent group was no significant difference within those groups.4. The mean expression of miR-127-5p in lymph node metastasis group negative and positive group of primary CISCC was 1. 3070±0.05912 and 1. 1988±0. 12589; the mean expression of miR-218 in these two groups was 1. 4641±0. 07449 and 1.2988±0.27536. Expression of the two genes was no significant difference between two groups (P>0.05).5. Expression of miR-127-5p in non-vascular invasion group and vascular invasion group was no significant difference between two groups (P>0.05). The mean expression of miR-218 in these two groups was 1. 3583±0. 01619 and 1. 2431±0. 0708, decreased miR-218 was significantly associated with vascular invasion (P=0.039).CONLUSION1. Manual microdissection was an effective method for collecting target cells from cervical tissue.2. Paraffin-embedded tissues were abundant resources for microRNA research.3. The expression of let-7a-1,let-7b and let-7c in cervical squamous cell carcinoma tissues was no significant difference compared to matched normal cervical tissues.4. The expression of miR-127-5p and miR-218 in cervical squamous cell carcinoma was no significant difference compared to matched normal cervical tissues.5. The expression of miR-127-5p was not significantly associated with neoadjuvant chemotherapy, FIGO stage, tumor size, pathological type, differentiation, deep muscular invasion, vascular invasion and recurrence in cervical caner.6. Increased miR-218 expression was significantly associated with vascular invasion (P=0.039). miR-218 expression was not significantly associated with neoadjuvant chemotherapy, FIGO stage, tumor size, pathological type, differentiation, deep muscular invasion and recurrence in cervical caner.7. Correlation between miR-127-5p,miR-218 and lymph node metastasis was undetermined, requiring larger sample size. |
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