Proteomics Study Of A Model Of Idiopathic Hyperoxaluria Rats In Its Liver

Objective To evaluate the feasibility of two-dimensional gel electrophoresis liver in ratswith idiopathic hyperoxaluria and labeling before DIGE, and to lay experimentalfoundation for DIGE.Methods 3 male rats with idiopathic hyperoxaluria as study group,3 normal ones ascontrol. The liver of rats with idiopathic hyperoxaluria and the normal liver tissue wereexamined by two-dimensional gel electrophoresis (2-DE) and labeling test.Results Six Satisfying two-dimensional electrophoregram and one SDS-PAGEelectrophoregram were obtained.Conclusions The mobility shift of the protein spots is stable in the six two-dimensionalelectrophoregrams and the protein extract is compatible with DIGE fluorescent dyes.Thestable repeatability and feasibility of DIGE is ensured.