| Objective: To explore the cure mechanism of recuperating-heartgranule on arrhythmic by the study of observing the effect of ECG,blood-serum; pathomorph of cardiac muscle, cardiac muscle cell of cx43proteinum,andATP sensitivity potassium channels when it affect experimentarrhythmic rat and rabbit.Method: The use of Bacl2, aconitine, coronary artery ligation in ratsinduced by rapid methods such as three kinds of arrhythmia, as well asexperimental animal models in rabbits fed high-fat combination ofintravenous epinephrine to copy the methods of rapid coronary heartarrhythmia model,Ⅱobserved ECG arrhythmia occurred, duration, changesin lipid content,Pathomorphologic aorta, as well as institutional changesSupermicro, Immunohistochemical detection of myocardial cx43 proteinexpression; RT-PCR determination of the impact of channel KATPmRNA.Results: 1.ECG showed: recuperating-heart granule can delay bacl2due to the occurrence of tachycardia in rats time to speed up the recoverytime electrocardiogram, Compared with blank control group significantdifference (P＜0.01)。Can delay the aconitine-induced arrhythmia in rats thetime to reduce the duration, and other group differences were significant (P＜0.01); Coronary artery ligation in rats can delay the emergence ofarrhythmia time, significantly shortening the duration of arrhythmia,significantly reduced the incidence of VT, compared with the control groupwere statistically significant differences (P＜0.01), Propafenone group witha considerable role, Can delay the emergence of coronary heart diseasearrhythmias time, reduce the duration and control group recuperating-heartgranule and recuperating-heart granule treatment group, model group, thedifference was statistically significant ((P＜0.01)), Compared with theamiodarone group was no significant difference (p＞0.05)2.Lipids assay showed: recuperating-heart granule model control group and compared to the control group, TG, CHO, ApoB were significantlydecreased, the difference was statistically significant (P＜0.05), HDL-C,ApoA has increased, the difference was statistically significant ( P＜0.05).3.Light microscope: recuperating-heart granule control group with mildreduction in aortic endothelial cells, intimal thickening than intimal lipiddeposition under, vascular smooth muscle disorder with mild changes in lightthan the other groups (except the blank group).4.Electron microscope: recuperating-heart granule control groupaortic endothelial cells in the lower surface of lipid deposition material, cellstructure is not clear, in the muscle fiber membrane with the rules ofcollagen fibers and cell protruding intertwined than the other group (exceptthe blank group) significantly reduce the injury.5.Immunohistochemistry showed: recuperating-heart granule controlgroup aizoon Cx43 expression was significantly higher than the same coloreddot size, the more uniform distribution. The results of semi-quantitativeimage analysis of recuperating-heart granule cx43 expression of controlgroup increased, and the blank group, model group, particle aizoondifference between the treatment group was statistically significant ((P＜0.01)), compared with the amiodarone group difference was not statisticallythe meaning of ((P＞0.05)).6.RT-PCR detection showed that: recuperating-heart granule treatmentgroup and control group aizoon particles, amiodarone group compared withthe model control group KATP mRNA /β-action mRNA ratio significantlylower, the difference was statistically significant (P＜0.01),recuperating-heart granule control group and the amine iodine ketone groupwas no significant difference (P＞0.05).Result:1. recuperating-heart granule of Bacl2, aconitine and coronaryartery ligation induced experimental arrhythmia arrhythmia and coronaryheart disease have a good Antagonism.2. recuperating-heart granuledyslipidemia particles can be improved to improve atherosclerosis and ultrastructural abnormalities pathomorphology to play anti-atherogenicrole.3, recuperating-heart granule may be increased 43 rabbit myocardial gapjunctional protein expression, reduced ATP-sensitive potassium channelKATP mRNA expression, prevention of coronary heart disease has played therole of rapid arrhythmia. |
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