| Sorghum belongs to genus sorghum,family Oryza,which is a one-year herb plant.It contains many chemical components such as proanthocyanidins,and possesses widely nutritional and pharmacological actions.The paper aimed to study the proanthocyanidins of sorghum.The main conclusions are as follows:1.Experiments were conducted to optimizing the extraction condition for extracting proanthocyanidins from sorghum grain.Results showed that ethanol concentration had the biggest effect to the extraction,followed by refluxing extraction time,material-liquid ratio and refluxing extraction temperature orderly.The optimized extraction conditeion is ethanol concentration of 70%,extraction temperature of 80℃,material-liquid ratio of 1:10 and extracting duration of 0.5 hour,two times.2.The proanthocyanidins was purified and condensed by diluting through macroporous resin AB-8.Collecting 30%ethanol eluting fraction,the proanthocyanidins content was 86.47%.After the polyamide resin purification,.the proanthocyanidins content was 79.62%.3.The extract derived before was purified by High-Speed Copunter-Current Chromatography(HSCCC).Three compounds were obtained and analyzed by HPLC-TOFMS;they were esteemed to be catechin,dimmer- catechin,and trimer- catechin.4.Total antioxidant activity was measured using animal model.Ageing rats were oral administrated with polymide extract,AB-8 resin extracts and 70%ethanol extracts, respectively.The level of MDA of all groups decreased significantly(p<0.05).The activities of GSH-px and SOD enhanced significantly(p<0.05) of all groups too.5.Lung cancer animal model was constructed by Lewis lung cancer cell intraperitoneal injection.And sorghum extracts were oral administrated with three levels,which were 1,2 and 4 g/kg body wt,respectively,for 20 days.The inhibition rate of high dose was 17.59%(p<0.05);and the trrans-inhibition effect of high,medium and low dose were 80.56%(P<0.01),72.12%(P<0.01) and 61.1%(P<0.01),respectively.It could conclude that the sorghum extracts could inhibited cancer cell proliferation in a dosage manner. 6.VEGF immune histochemistry terst showed that the extracts of sorghum could significantly inhibited the expression of tumour cell at high,medium and low concentration.7.Near-infrared reflect spectroscopy(NIRS) technology was applied on the determination of the procyanidins content of sorghum.The main purpose is to investigate the feasibility to construct the mathematic model for procyanidins content of sorghum with partial least-squares(PLS) test.Regression model was evaluated in terms of root mean square error of cross-validation(RMSECV),the root mean square error of prediction(RMSEP) and inner/outer cross-validation test correlation coefficient(R~2).The inner corss-validation test correlation coefficient(R~2) is 0.8657 and RMSECV is 0.277,outer corss-validation test correlation coefficient(R~2) is 0.8919 and RMSECP is 0.312.The results showed that NIRS,a well-established and widely applied technique could be applied to determine procyanidins content in sorghum rapidly and nondestructively.8.Materials about resource of sorghum proanthocyanidins showed that proanthocyanidins contents covered from 0.01%to 4.1%.Sorghums produced in Heilongjiang,Hebei,Shaanxi,Shandong,Anhui,Sichuan,Guizhou,Yunnan and Guangxi Province contain more than 1.5%proanthocyanidins.Yunnan,Guizhou and Sichuan sorghums contain more than 2.0%proanthocyanidins in average.Sorghums resources from five Provinces and zone contain proanthocyanidins less than 1.0%,they are Xinjiang,Jilin, Liaoning,Mongolia,Beijing and Henan,and sorghum produced in Xinjiang contains the lowest proanthocyanidins by 0.025%.To conclude,sorghums form northern place of production contain higher proanthocyanidins than southern zones. |
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