Clinical And Experimental Study On Child Anorexia By Taken KAI WEI JIN SHI Ultrafine Particle

ObjectiveTo observe the clinical effect for child anorexia,the renovation of ultramicrostructure of sinuses ventriculi mucosa and the adjustment of appetite center in juvenile rat model with anorexia by taken KAI WEI JIN SHI ultrafine particle.It was expected to reveal possibility mechanism of treating anorexia.Methods1.108 patients with child anorexia by feeded respectively with KAI WEI JIN SHI ultrafine particle,KAI WEI JIN SHI TANG and JIAN WEI XIAO SHI PIAN were divided into three groups in random.2.Juvenile rat models with PI XU SHI JIAN anorexia were established by feeded special forage and DA HUANG Tea,and simulating the cause of child anorexia.3.Ultrastructure of sinuses ventriculi mucosa of juvenile rat models with anorexia was observed by transmission electron microscope(TEM). Mitochondrial in epicyte and oxyntic cells and the changes of quantity,area, perimeter,major axis,minor axis,roundness number of chief cell zymogen grain were analysised with quantimet.β-endorphin(β-EP) in center and peripheral blood,sinuses ventriculi mucosa,Octa-cholecystokinin(CCK-8) and it's receptor were examined with radioimmunoassay(RIA). Results1.It was shown that the total effective rate were over 86%within one cause of treatment and there was no statistical significance in the three groups. Symptomes and physical sign in the group feeded with KAI WEI JIN SHI ultrafine particle was improved predominantly.Food-intake and body weight were increased simultaneously.It showed a statistical significance by compared with matched group(P＜0.05 or P＜0.01).2.It was shown that food-intake of juvenile rat models with anorexia were 40～50%lower than normal control,and weight 10%～15%lower before feeded with KAI WEI JIN SHI ultrafine particle(P＜0.01).It was righten after treatment and shown no statistical significance by compared with matched group(P＞0.05).3.It was shown that Mitochondrial in epicyte and oxyntic cells of sinuses ventriculi mucosa of juvenile rat models with anorexia,and the quantity,area, perimeter,major axis,minor axis,roundness number of chief cell zymogen grain changed obviously.There was a statistical significance by comparing with normal control(P＜0.05 or P＜0.01),and no significance in ultrastructure between the group feeded with KAI WEI JIN SHI ultrafine particle and normal control.β-EP in hypothalamus,plasma and sinuses ventriculi of juvenile rat models with anorexia were lower than normal control(P＜0.05 or P＜0.01), CCK-8 were higher(P＜0.05 or P＜0.01).Statistic above include it's acceptor were changed into normal after being feeded with KAI WEI JIN SHI ultrafine particle(P＜0.01).Conclusion1.It was suggested that KAI WEI JIN SHI ultrafine particle was effective for child anorexia,which taken conveniently,quickly and economically.2.It was suggested that juvenile rat models with PI XU SHI JIAN anorexia established by feeded with special forage and DA HUANG tea to simulating the cause of child anorexia was stable and credible,which was useful for study on pathogenesis and pharmacodynamics of child anorexia.3.It was suggested that the occurrence of anorexia concerned with the abnormal ultrastructure of sinuses ventriculi mucosa of juvenile rat models with anorexia.KAI WEI JIN SHI ultrafine particle might intervent the abnormal ultrastructure effectively,promote synthesize and excretion ofβ-EP in hypothalamus,plasma and sinuses ventriculi of juvenile rat models with anorexia,increse acceptor,represse CCK-8 and it's acceptor.It was conclused that KAI WEI JIN SHI ultrafine particle be ability to renovate sinuses ventriculi mucosa and adjust appetite center.It might be mechanism treating anorexia.