Effects Of Huangqi Fuzheng Tang On Immune System And The Initial Exploration Of Its Mechanism

Chapter 1 Enhancement of immunity in the Immunosuppressive Mice by Different Dosage of Huangqi Fuzheng TangObjective:To study if Huangqi Fuzheng Tang could enhance the immunity in the immunosuppressive mice and find out the best dosage.Methods:Sixty male Kunming mice were divided into 6 groups: normal control group,model group,9g/kg,18g/kg,36g/kg and 72g/kg Huangqi Fuzheng Tang treatment groups.Set up the immunosuppressive animal model by using hydrocortisone.Different dosages of Huangqi Fuzheng Tang apozema were given to the treatment groups by intragastric administration once daily for 14 days.The normal control group and the model group received the same volume of normal saline.The contents of serum hemolysin and IL-2 of the mice were measured.Results:After giving hydrocortisone,compared to that of the normal control group,the serum hemolysin and IL-2 of the mice were decreased obviousely(P＜0.05).Huangqi Fuzheng Tang could elevate serum hemolysin and IL-2 when the dosages were 18g/kg,36g/kg and 72g/kg.The effect of 36g/kg was the strongest,the disparity had statistical significance.Conclusion:1.The immunosuppressive mice model was set up successfully by giving hydrocortisone intraperitoneal injection.2.The most effective dosage of Huangqi Fuzheng Tang was 36g/kg. Chapter 2 Enhancement of Immunity in the Immunosuppressive Mice by Huangqi Fuzheng TangObjective:To study the enhancement of immunity in the immunosuppressive mice by using Huangqi Fuzheng Tang.Methods:Forty mice were divided into 4 groups:normal control group,model group,Huangqi Fuzheng Tang(HFT) group and Yupingfeng San(YPF) group.Set up the immunosuppressive animal model by giving hydrocortisone intraperitoneal injection.Huangqi Fuzheng Tang and Yupingfeng San apozema were given to the mice of the treatment groups by intragastric administration once daily for 14 days.The normal control group and the model group received the same volume of normal saline. After 14 days the apoptosis of the spleen cells were assayed by using TUNEL staining.Bcl-2 and Bax proteins in the spleen were assayed by immunohistochemistry.The contents of hemolysin,IL-2,INF-γin the mice serum and the expression of CD3~+,CD4~+,CD8~+ in the peripheral blood lymphocytes were measured.RT-PCR and Realtime PCR were used to evaluate the mRNA of INF-γ,IL-4,T-bet and GATA-3 in the spleen cells.Results:1.Compared to that of the normal control group,the apoptosis of the spleen cells in mice of the model group was increased significantly(P＜0.05).After treatment,the apoptosis of the spleen cells decreased significantly(P＜0.05),but there was no difference between HFT group and YPF group(P＞0.05).2.The mice of the model group had less Bcl-2 positive cells and more Bax positive cells in the spleen than that of the normal group mice(P＜0.05).After treatment,Bcl-2 positive cells increased and Bax positive cells decreased in the mice of HFT group and YPF group(P＜0.05),but there was no statistical difference between the two groups.3.The serum hemolysin in the mice of the model group were lower than that of the normal group mice(P＜0.05).After treatment,the serum hemolysin elevated obviously in the mice of the HFT group and YPF group,but there was no statistical difference between the two groups.4.Compared to that of the normal control group,the model group had a lower level of serum INF-γ,but a higher level of serum IL-4(P＜0.05).Huangqi Fuzheng Tang could obviously elevate INF-γ,and descent IL-4,acted more effective than Yupingfeng San(P＜0.05).5.Huangqi Fuzheng Tang could elevate the ratio of CD4~+/CD8~+T cells,and there was statistical difference between Huangqi Fuzheng Tang and Yupingfeng San(P＜0.05).6.Both the RT-PCR and Realtime PCR revealed the Huangqi Fuzheng Tang could enhance the mRNA expression of INF-γ,and T-bet, but reduce the mRNA expression of IL-4 and GATA-3.And there was statistical difference between Huangqi Fuzheng Tang and Yupingfeng San.Conclusion:1.Huangqi Fuzheng Tang could enhance the humoral immunity,but there was no statistical difference between the two.2.Huangqi Fuzheng Tang could enhance the cellular immunity, balance the ratio of Th1/Th2 cytokines,and acted more effectively than Yupingfeng San in some aspects.3.Huangqi Fuzheng Tang regulated the Th1/Th2 cytokines might through regulating the transcriptional factor T-bet and GATA-3. Chapter 3 Immunomodulatory and Anti-oxidative Activity in the Elderly by Huangqi Fuzheng TangObjective:To investigate whether taking Huangqi Fuzheng Tang apozema daily could enhance immunity and have anti-oxidative effect in the elderly.Methods:Sixty elderly(age＞60 years) volunteers participated in this program.They were divided randomly into two groups:Huangqi Fuzheng Tang(HFT) group and the Polysaccharide nucleic acid fraction of BCG(BCG-PSN) group.Volunteers in the HFT group took Huangqi Fuzheng Tang apozema 300ml/day,and the BCG-PSN group received BCG-PSN injection 1mg i.m twice a week.The treatment continued for 4weeks.Before and after the treatment the immunological parameters of the subjects' blood were examined.The parameters included serum IgG,INF-γand IL-4,expression of CD3~+,CD4~+,CD8~+,CD56~+in the peripheral blood lymphocytes.Serum concentration of SOD,total anti-oxidative capability and MDA were also measured.Results:1.The rate of dropping out was higher in the BCG-PSN group than that of the HFT group(P＜0.05).2.After the treatment,subjects'serum IgG elevated significantly in the HFT group than that before treatment(P＜0.05).But there were no statistical differences between the two groups.3.After the treatment,subjects'serum INF-γincreased and IL-4 decreased significantly in the HFT group than that before treatment(P＜0.05).But there were no statistical differences between the two groups.4.After the treatment,the ratio of CD4~+/CD8~+T cells of the elderly was higher than that before treatment(P＜0.05).And in HFT group that increased more obviously than that of BCG-PSN group(P＜0.05).5.Huangqi Fuzhegn Tang could elevate the CD56~+ cells in the blood, statistically different from before the intervention.But there was no statistical difference between Huangqi Fuzheng Tang and BCG-PSN.6.The levels of serum SOD and the total anti-oxidative capability in the HFT group increased significantly than that before the therapy(P＜0.05).But in the BCG-PSN group the levels of SOD and the total anti-oxidative capability were not changed before or after the treatment(P＞0.05).7.The subjects'serum MDA were lower than that before in the HFT group(P＜0.05).But in the BCG-PSN group there were no differences before or after the treatment(P＞0.05).Conclusion:1.Huangqi Fuzheng Tang could enhance the cellular immunity and the humoral immunity in the elderly,increase the amount of NK cells.2.Huangqi Fuzheng Tang could balance the ratio of Th1/Th2 cytokines,and had a better effect than BCG-PSN.3.Huangqi Fuzhegn Tang had anti-oxidative effect and improved the oxidative stress,that might be one of the anti-immunosenescence mechanisms.