The Effect Of Adriamycin Nephropathy Rats Kidney Glomeruls' Charge Barrier And Nephrine By The SHENTELING Adding Prednison

Objective:1.To study a method of improving the model of adriamycin nephropathy and establish a stable rats' model of adriamycin nephropathy.2.To observe the effect to barrier and nephrine on rats' model founded by adriamycin and discuss the mechanism for treating NS by animal's experiments.Methods:1.To found rat' s nephropathy models by being received a single tail-vein infection of 7.5mg/kg adriamycin.24 hours urinary protein,ALB,BUN,Scr,Chol Were detected.Kidney tissue were detected with microscope and electronmicroscope.2.Rats were divided randomly into control group(10 rats)and making models group(30 rats).Then,nephropathy rat' s models were founded with a single tail-vein infection of 7.5mg/kg adriamycin.Making models group were divided randomly into model group,Prednisone group and Shenteling adding Prednisone group 14 days after making models,10 rats each group.Drugs were given to rats of Prednisone group and Shenteling adding Prednisone group,then,all rats were detected 24 hours urinary protein,ALB,BUN, Scr,Chol;barrier with PEI,AB and nephrine with RT-PCR.Results:1.The level of urinary protein and Chol increased gradually,urinary protein and Chol set at peak,on the other hand,ALB declined,the level of ALB was set at the lowest peak in experimental group in 28^th.At the same time,the level of BUN and Scr elevated not too obvious.Glomerular form had no obvious alterations with microscope and obvious alterations with electronmicroscope.There had no alterations in control group.2.Following drugs given,the level of urinary protein increased gradually and set at peak in model group in 28^th,at the same time,the level of urinary protein in Prednisone group and Shenteling adding Prednisone group was belower than model group.Comparing with Prednisone group,the level of urinary protein,ALB,Chol,AB dyeing rate of glomerular and barrier had statistically significant in Shenteling adding Prednisone group.Barrier altered with electronmicroscope in model group.Conclusions.1.It can found the rats' model about adriamycin nephropathy successfully with a single tail-vein infection of 7.5mg/kg adriamycin.It also can shorten experimental times and it' s character is agreement with human NS. 2.Foot processes' uhrastructure changes of adriamycin nephropathy rats is ealier than urinary protein and it' s severity is agreement with urinary protein excretion.3.Both barrier damage and nephrine expression decrease are the main etiology of a lot of proteinuria.4.One of the effective mechanism of Shenteling adding Prednisone is repairing barrier damage and increasing nephrine expression,it' s therapentical effect is better than Prednisone onely.