| Objectives1. Investigating the mechanism of Atopic Dermatitis (AD) more deeply through measuring IgE,IL-5,IL-12 and IL-13 of the patients with AD.2. Observe changes of the scores of SCORAD index before treatment and 3 months after treatment of the formula, to value therapeutic effect of advisor's formula of clearing away heart-fire and hilling.3. Measuring IgE, IL-5, IL-12andIL-13of the patients with AD using the ELISA technique before and after the treatment for 3 months respectively. Then compare with 19 healthy controls, in order to investigate the possible mechanism of treatment. It is good for finding its possible mechanism to direct our clinical practice and to make basis of the development of new Traditional Chinese medicine.Method19 patients diagnosed atopic dermatitis are selected to this clinical trial. Scores of SCORAD index are collected before treatment and 3 months after treatment respectively. The level of IgE,IL-5,IL-12 and IL-13 in the serum of both the patients and health controls were detected by ELISA. Results1.19 patients diagnosed atopic dermatitis were admitted to the clinical trial and treated with formula of the clearing away heart-fire and hilling. According to evidence to adjust Chinese herb. SCORAD scale was used to evaluate clinical therapeutic effect. After treatment, scores of SCORAD index decline. So it is demonstrates that the method of clearing away heart-fire and hilling is used to AD patients.2. Before treatment, IgE in serum of the patients was higher than those of the healthy controls (P<0.01), compared with those before treatment. IgE had much change after treatment (P<0.05).3. The value of IL-5 before treatment of AD patients was more than that of healthy people. The statistic significance was also demonstrated (P< 0.05). After treatment of the formula of clearing away heart-fire and hilling, the value of level of IL-5 after treatment was lower than that before treatment with statistical significance (P< 0.05).4. The level of IL-12 of AD patients before treatment was lower than that healthy people. The difference is statistically significant (P> 0.05). After treating with the formula of clearing away heart-fire and hilling, the value of IL-12 arised with statistical significance (P>0.05).5. The values of IL-13 among healthy people, before treatment and after treatment are nearly the same (P>0.05).Conclusions1. After treating with formula of the clearing away heart-fire and hilling, the scores of SCORAD index decline. So it is demonstrates that the formula of clearing away heart-fire and hilling is used to AD patients.2. Treatment with the formula of clearing away heart-fire and hilling, the level of the IgE was decreased (P<0.05), we speculate that it may be one of therapy mechanism of AD.3. After treating with formula of the clearing away heart-fire and hilling,the level of IL-5 declined (P<0.05), the level of IL-12 increased (P>0.05), we speculated that the unbalance of expression of Thl/Th2 cytokines was improved. |
PDF Full Text Request