Association Between H.pylori-HSP60 And Coronary Atherosclerosis

Part IAssociation between helicobacter pylori-heat shock protein60 and acute coronary syndromeObjective To investigate the possible association between helicobacter pylori heat shock protein 60 and acute coronary syndrome (ACS). Methods heat shock protein 60 was assessed by ELISA in 62 patients with ACS and 24 controls. We measured plasma CRP levels by immuno-nehelomitery. Results Prevalence of heat shock protein60 was higher in ACS patients than in controls (P<0.05). The patients of 3-lesions group also had a higher prevalence of HSP60 than 1-lesion and 2-lesions group. HSP60 was significantly higher in 2-lesions than 1-lesion group (P<0.05). Moreover, Prevalence of hsCRP was higher in ACS patients than in controls (P<0.05). Conclusion The association between increased hsCRP and ACS was close. HSP60 seemed not only to be related to ACS but also to be more related to pathological changes of coronary artery.Part IIEffects of Anti-Helicobacter Pylori Infection on Prognosis in Acute Coronary Syndrome Patients.Objective To evaluate the effects of anti-Helicobacter pylori (Hp) on coronary events and the serum levels of inflammation substances in acute coronary syndrome patients. Methods 60 ACS patients with HP infection were randomly divided into two groups, Both groups were administered with routine therapy. The trial group was additionally administered with drug of lizhu(12 days). Clinical efficacy and changes of HSP60 and hsCRP, incidence of coronary events in two groups were observed before and after treatment. Results The follow up period was one month. The incidence of the coronary artery events were 16.7% in treatment group and 40% in control group, Among them, readmission with angina were 6.7% in treatment group compared to control group (16.7%).The serum levels of HSP60 and hsCRP in treatment group were distinctly lower than those in control group after treatment (P<0.05). Conclusion Hp eradication can significantly decrease the incidence of myocardial infarction and readmission with angina and the demand for PCI/CABG. Anti-Hp infection has much curative effect in the treatment of ACS patients. PartⅢEffects of Helicobacter pylori-HSP60-induced Regulatory T cell on the progression of atherosclerotic plaque in miceObjective To explore the induction of Helicobacter pylori-HSP60-induced CD4+CD25+T cell in vitro and its effect on the formation of atherosclerotic plaque. Methods Immature dendritic cells were extracted and induced from mouse bone marrow and then used to induce Helicobacter pylori-HSP60 CD4+CD25+T cells in vitro. After infusing the CD4+CD25+T cells into homogenous mice, the size of atherosclerotic plaques was measured. Results COompared with control group, the expression of CD80(43%) and CD86(41.1%) on aspirin-treated dendritic cells was down-regulated. Immature dendritic cells induced more specific regulatory cells (13.0±1.94%) than the mature ones. These cells significantly suppressed the proliferation. The size of atherosclerotic plaques (2.37±0.96mm2) in treated mice were smaller than untreated ones (P<0.05). Conclusion Immature dendritic cells can be used to induce HSP60 specific regulatory T cells in vitro and the latter also can inhibit the progression of atherosclerosis.