| The topics is to be perspective from animal experiments in mouse behavior, neurotransmitter, neurotrophic factor, genes, etc., and to explore the pathogenesis of post-stroke depression and the mechanism of antidepressant effect of Chinese medicine, and to provide a theoretical basis for clinical treatment and reference.1.Experiment 1 for the Chang Yu Xiaoyaosan right PSD study the impact of their behavior in rats.In this experiment, the combined treatment prepared a mouse model of post-stroke depression. Observed in behavioral studies,Making model mouse in each group after 1-2 weeks were beginning to show significant depressive symptoms, manifested as a lack of interest in pleasure, crouching not move, easy to despair, irritability, significant weight loss. Sugar consumption and other experiments also proved the success of modeling. It was found that Chang Yu Xiaoyaosan can be significantly improved depressive symptoms in mouse, increasing body weight of mouse PSD, to increase the sugar consumption in mouse, reduce PSD swimming immobility time in mouse, and immobility time in tail suspension.2.Experiment 2 is to explore the PSD brain monoamine neurotransmitter changes, and observe the effects of Chang Yu Xiaoyaosan on brain Delivery of monoamine in mouse. Animal handling and preparation of models with experimental one, behavior after the end of the experiment, the mouse in each group were decapitated brain isolated from the ice stage frontal cortex, hippocampus and placed in-80℃refrigerator. The results of modeling of monoamine transmitters in each group than those in normal group dropped sharply, ChangYuXiaoYaoSan can significantly improve the PSD mouse brain NE,5-HT, DA and other monoamine neurotransmitters in the level of quality.3.Experiment 3 is for the pairs of post-stroke depression in mouse BDNF, GFAP and other neurotrophic factors studied. It was found that, PSD mouse there exists an obvious BDNF, GFAP expression of neurotrophic factors such as decreased expression,proved that the incidence of post-stroke depression and BDNF, GFAP expression of neurotrophic factors such as closely related. Chang Yu San Yu San mechanism may be related to the clinical antidepressant effect is by enhancing post-stroke depression in patients with cerebral frontal cortex, hippocampus and many other parts of BDNF, GFAP and other neurotrophic factor expression.4.Experiment 4 is to study the effect of c-fos,TH gene expression in rats.The experiment was found, PSD mouse there is significant expression of c-fos increased and TH expression levels decreased. Chang Yu San can quickly inhibit c-fos expression, increased TH gene expression.
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