Analysis Of Oryza Sativa Arginase Gene Transcription Patterns And Functions

Improving nitrogen use efficiency (NUE) would play beneficial roles on rice yield increment. However the absorption, storage and utilization of nitrogen are complex processes involving in coordination function of carbon and other metabolims. Relevant information about it is scarcity at present. For many years of selection of mutants, we have obtained a nitrogen nutrient defect mutant by a recessive gene.In this sub jet, arg mutant obtained by tetraploid anther culture was used. And further study will be done about the rice arginase gene transcription patterns and functions of regulation networks about fatty acid metabolism.Firstly, the analysis of the transcription patterns of rice arginase gene indicated that rice OsARG gene might participate in the regulation of drought and salt depended on ABA. Rice OsARG gene was induced by NaCl and PEG, but not by Mannitol and cold. Ane rice OsARG gene was induced by ABA, but not by Ethylene, GA,6-BA, BR, NAA, IAA, IBA, MeJA, SA, GR24.Secondly, our results indicated that the nitrogen metabolism regulated by OsARG gene was closely related to fatty acid metabolism during the development of stamens. Using GC-MS analysis showed that the content of fatty acid in ar,g-mutant is much lower than in wild type. Using RNA-seq analysis showed that deletion of OsARG gene effected many genes expression of fatty acid metabolism. Over-expression three gene LOC_Os08g20200, LOC_Os12g13930and LOC_Os03g11760in mutants indicated that the To transgenic plants partly recovered thephenotyp of mutants, but further study should be done about T1transgenic plants.Aim to elucidate the mechanisms of nitrogen and fatty acid metabolisms of OsARG, and to provide the new guidance.