Development And Application Of Surface Plasmon Resonance Imaging Systems

Surface Plasmon Resonance (SPR) imaging has been widely applied for biomolecule interaction analysis (BIA). The technique is real-time, label-free and highly sensitive. Thus it is advantageous to provide kinetic information about biomolecule interaction. In this assay, the performance of the SPR system has been improved in many ways, including:First, an angle-scanning SPR imaging system was fabricated based on a novel angle-scanning platform. In terms of the mechanical design, the SPR imaging system was based on a high-resolution linear piezoceramic motor and a rhombic motion converter. The angular resolution of the angle-scanning platform was10-4degree. On the aspect of the software development, the drive system of the electronic elements, the operation platform was completed using VC++. The ultimate precision of the SPR imaging system was1×10-3degree.Second, the system was used to detect mismatched bases in caspase-3DNA with high throughput. The DNA probe arrays were fabricated by a home-made spotter, and hybridized with the target DNA. The results displayed that the SPR sensor could carry out comparison and differentiation between the caspase-3DNA sequences. A single mismatch in DNA could be distinguished. The detection limit was25pmol/L. Based on the platform, an SPR imaging system for cell imaging was fabricated to take both the SPR images and the images of the cells in a normal view. The system had two imaging units:one was used to capture regular SPR images of the cells and the other took the cell images in a top view. The comparison of the two images would give more reliable and detailed information of the sensor surface and excluded many disturbing factors. The system was used to monitor the cancer cell HepG2.Third, a power-free flow injection microfluidic system was developed. In the system, the liquid sample was driven by its gravity forces. The gravity-induced flow injection system was able to maintain a relatively stable flow rate with manual operations. The device was used to obtain the kinetic information of the hybridization process between complementary DNAs. Then a handheld SPR imaging device was developed for on-site detection...