| Objective:Acute rhinosinusitis(ARS) is a common nasal disease with high incidence.My teacher, Mr. Xiong Dajing, who based on the foundation of traditional Chinese medicine, summarized as improving Qi’s flowing based on regulating Shao-yang channel, and through opening orifice regulating function of Live and Gall-bladder, tonifying Correct Qi as the core of his cademic thought after more than40years clinical practice, and exerted it throughout his treatment on otorhinolaryngology diseases.Bidouyan-oral liquid (sinusitis oral liquid)which from Ji Lei Kai Qiao Tang has reliable treating effect on allergic.We observe the effect of Bidouyan-oral solution on beta-defensin in nasal mucosa of ARS rats modal,meanwhile, study the mechanism of action about it to Bi Yuan by animal experiment, then to guide the clinical medication more accurately.Methods:Group the healthy SD rats into the following six parts randomly:blank group, model group, Ji Lei Kai Qiao Tang low-dose group, Ji Lei Kai Qiao Tang middle-dose group, Ji Lei Kai Qiao Tang high-dose group and Amoxicillin and Clavulanate Potassium group. According to the method reported by YE Jing, we made the Acute Rhinosinusitis model.In the following seven days we drenched the blank group and the model group5ml·kg-1·d-1with the sterile water (SW) for7days, meanwhile the other four groups with corresponding medicine. All these days we observed the changes of the rats about the pH value, body temperature, and body weight, especially the nose symptom and secretory volume. After seven days,6rats randomly choosed in each group to exam ph of nasal secretions, leucocyte count(WBC) and the percent of neutrophils (N%)of peripheral blood cells,then we killed the rats to get nasal mucosa to observe the morphologic changes of it under light microscope. AT the time.SP immunohistochemistry method was used to test BD-1,BD-2,BD-3in nasal mucosa, and RT-PCR(real time polymerase chain reaction) methods was used to test BD-2mRNA.Results:1.Comparing with the blank group, the pH value of the model group’s nasal cavity secretion substance has improved significantly (P<0.01). The nasal cavity secretion substance’s pH values of all the therapy groups have declined, especially the Ji Lei Kai Qiao Tang high-dose group and Amoxicillin and Clavulanate Potassium group.2.Comparing with the blank group, WBC and N%of the model group has improved significantly (P<.0.01). WBC of all the therapy groups have declined significantly (P<0.01), especially the Ji Lei Kai Qiao Tang high-dose group and Amoxicillin and Clavulanate Potassium group. 3. MOD for BD-1were no significant difference between all groups(P>0.05).4.Comparing with the blank group,MOD for BD-2of the model group has declined(P<0.05).After seven days medication, except the Ji Lei Kai Qiao Tang low-dose group, the MOD for BD-2of all the therapy groups have improved(P<0.05) comparing with the model group.But BD-2mRNA were no significant difference between all groups(P>0.05).5.Comparing with the blank group, MOD for BD-3of the model group has improved(P<0.05).After seven days medication, the MOD for BD-3of the Ji Lei Kai Qiao Tang high-dose group and Amoxicillin and Clavulanate Potassium group have improved significantly(P<0.01).6. Using the light microscope,Significant pathological changes of acute inflammation were observed in nasal membrane of the model group,After seven days medication,except the Ji Lei Kai Qiao Tang low-dose group,the nasal membrane of all the therapy groups have returned to normal gradually especially the Ji Lei Kai Qiao Tang high-dose group Amoxicillin and Clavulanate Potassium group.Conclusion:1.By falling the Nasal discharge pH value to normal, Bidouyan-oral liquid can make the internal environment concordance of the nasal cavity.2.MOD for BD-1were no significant difference between all groups(P>0.05).The result suggest that BD-1may paly a constitutive role in nasal defenses.Bidouyan-oral liquid can not induce protein expression of BD-13.The middle and high dose Bidouyan-oral liquid can strengthen the protein expression of the nasal mucosa BD-2,to improve the anti-infection ability of the organism.4.During the acute inflammation, the protein expression of the nasal mucosa BD-3can be strengthened.The result can not suggest that Bidouyan-oral liquid can change protein expression of BD-35. Bidouyan-oral liquid can control the general and local inflammatory reaction in ARS rats,especially the high dose.We suppose that Bidouyan-oral liquid can clear gallbladder and purging heat improve Qi’s flowing based on regulating Shao-yang channel,disperse lung-qi and remove evil to open orifice.In this way,it can improve organism internal environment,strengthen vital qi to eliminate pathogenic factor. |
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