Clinical Observation Of Supermicro Ding Chuan Decoction On Thermal Asthma Syndrome And Effect Of Supermicro DingChuanDecoction On Neurogenic Inflammation Of Asthmatic Rat

Objective:To compare effect of different doses of supermicro DingChuan Decoction on pulmonary immune neurogenic airway inflammation of thermal asthma model rats, to investigate the curative mechanism, to explore effective dose. To observe clinical effect of supermicro DingChuan Decoction on adult and childhood asthma(thermal asthma),and to detect lung function changes of patients before and after treatment,to provide evidence for its clinical application.Methods:①health Sprapue-Demey rats are randomly divided into normal control group (group A), asthma model group (group B), DingChuan Decoction group (group C), low-dose supermicro DingChuan Decoction group (group D), supermicro DingChuan Decoction normal dose group (group E). To make ovalbumin asthma model with airway responsiveness determination to ensure successful.Group D and E are respectively to given supermicro DingChuan soup1/2dose and normal dose. To Observe NGF and SP and its mRNA expression in lung tissue of the rat models,and to determinate several positive coefficient and grey value of vision with microscopic image analysis system.②andomly90cases of adult and90cases childhood asthma patients are separately divided into3groups, control group are given aminophylline and budesonide therapy, besides the drugs DingChuan Decoction group are given DingChuan Decoction, supermicro DingChuan Decoction group are given supermicro DingChuan Decoction.To detecte EOS, IL-4and lung function level.before and after treatment.Results:The basic respiratory resistance (Re) of the five groups rats are similar both before and after with physiological saline stimulated,there is no statistical significance (P>0.05). After given10-40ug/kg of Ach excitation, the difference of asthma model group and normal control group is statistically significant (P<0.05). After given80-160ug/kg of Ach excitation, Re of asthma model group increased significantly (P<0.01). There is no difference between normal dose group and low dose group (P>0.05). NGF> SP of model group increased, the difference between model group and other groups is significant (P<0.01), there is no difference between the dose group, low dose groupand normal control group (P>0.05). average positive coefficient of NGF、NGFmRNA of lung tissue of Dingchuan Decoction group, normal dose group, low dose group rats is lower than that in the model group, the difference is statistically significant (P<0.01).Clinical study of adult asthma shows, the difference of total efficiency between Dingchuan Decoction group and the western medicine control group have no statistical difference, which shows the two have similar clinical curative effect(P>0.05). In relieving asthma and wheeze, there is no significant difference between groups (P>0.05). In the improvement of cough and expectoration symptom, there is significant difference (P<0.05), Dingchuan Decoction and supermicro Dingchuan decoction is better than western medicine control group. In spirit, appetite, thirst, tongue and pulse condition, there is no significant difference between the three methods (P>0.05). In the improvement of fever, complexion, toilet and other symptoms, there is statistically significant difference (P<0.05three), Dingchuan Decoction and supermicro Dingchuan decoction is better than western medicine control group. Are all improved after treatment in three groups of FEV1and PEFR than before treatment (P<0.05), as for treatment effect,there is no significant difference between Dingchuan Decoction group and Western medicine control group (P>0.05). After treatment, increased EOS in the peripheral blood of asthma of three groups is significantly decreased,with statistical significance (P<0.01), Dingchuan Decoction group and Western medicine control group show no statistical difference (P>0.05). The three groups after treatment can significantly decrease the IL-4level in serum of patients with asthma, there is statistically significant differences (P<0.01), Dingchuan Decoction group and Western medicine control group show no statistical difference (P>0.05).Clinical study on childhood asthma shows, total efficiency compared, difference is not statistically significant (P>0.05), and as for the clinical control rate, treatment group is obviously higher than that of the control group, the difference is statistically significant (P<0.05), as for the main symptoms and the total score points,there is significant difference between before and after treatment, as for the control group, in addition to the cyanosis, and other symptoms, there are significant differences in total integral comparison. By comparing difference of improved symptoms of each group (group t test), cough, sputum, flaring of nares, wheezing sounds have no difference (P>0.05); Dingchuan Decoction group and supermicro Dingchuan Decoction group are better than the control group in improving the wheezing, cyanosis (P<0.05). The total integral secondary symptom scores of the Dingchuan Decoction group, supermicro Dingchuan decoction group, are significantly different before and after treatment. In addition to fever, there are significant differences in total score of other symptoms before and after treatment. The comparison between the groups in improvement of symptoms, thirst, appetite, spirit of tongue, pulse,with no difference (P>0.05); fever, complexion, feces and urine are significantly different (P<0.05). Dingchuan Decoction group, supermicro Dingchuan decoction are better than the control group. Are all improved after treatment in three groups of FEV1and PEFR than before treatment (P<0.05), which shows that all groups can improve lung function. In the improvement of pulmonary function, Dingchuan Decoction group and supermicro Dingchuan Decoction group are better than western medicine control group (P<0.05).Conclusion:①The formation of the rat asthmatic model, has the advantages of simple operation, high success rate,and airway inflammation is clear ②he NGF, SP content in the lungs of sensitive asthmatic model rats increase. Airway mucosa, submucosa, muscle layer and pulmonary interstitial inflammation cells show significant NGF expression and localization, it can also be observed that NGFmRNA expression level is significantly increased of the site.③ingchuan Decoction and its supermicro substance can reduce the level of NGFmRNA expression of NGF and protein levels in lung of asthma model rats, which shows that it has the improvement effects on airway neurogenic inflammation.④he supermicro Dingchuan Decoction of1/2equivalent dose can reach the same effect of traditional decoction, can relieve the cough and phlegm, relieve asthma, can significantly improve lung function, reduce abnormal increased asthma eosinophils in peripheral blood and downregulate IL-4, superior to the western medicine control group⑤he supermicro Dingchuan Decoction has the same effect of control group in relieving asthma and wheeze. In the improvement of cough and expectoration symptom, Dingchuan Decoction and supermicro Dingchuan decoction are better than western medicine control group, which shows Dingchuan Decoction and supermicro Dingchuan decoction have better effect in relieving asthma and cough expectorant. ⑥he supermicro Dingchuan Decoction have more obvious effect in the treatment of children febrile asthma, wheezing, cyanosis and the fever, complexion, toilet and other symptoms, while have no obvious difference in the other symptoms. The total curative effect statistics are better than western medicine control group.⑦he supermicro Dingchuan decoction has good clinical effect in treatment of thermal asthma, and is safe...