Mongolian Medicine Wen Needle Therapy For Early Brain Injury Of Cerebral Ischemia Model Rats And The Effect And Mechanism Research

Cerebral Ischemic Stroke is one of the main types of Stroke. It has the characteristics of high incidence rate, high death rate and high disabled rate. Because of the characteristics mentioned above, Ischemic Stroke has become the most dangerous disease that has to be conquered. The study of Ischemic Stroke has also become an important subject in the basic medical and clinical medical aspects. This kind of disease belongs to the category of Hemiplegia. Mongolian medicine has its own recognition and theoretical system about this kind of disease, and according to long term practical experience, it has plenty of experience and unique way to treat the disease of Ischemic Cerebrovascular. Mongolian Medical Warm Acupuncture has the functions of improving blood-going, protecting Hectar in Sparta and improving blood-supplying to the brain. Thus It has become one of the treating methods to the syndrome and the brain infarctions caused by the lack of brain blood-supplying, and it has also the advantages of rapid, easy and easy-doing characteristics. This dissertation puts forward that Mongolian Medical Warm Acupuncture can reduce brain damage after Ischemic Stroke, promote the reparation of neural function, assume the brain protection to the metabolic of brain cells by clearing free-radicals, anti-oxide damage, and by protecting the release of Inflammatory cytokines. The dissertation also uses the research method of MACO-R to narrate the mechanism of Mongolian Medical Warm Acupuncture to the early anti-brain damage by Cerebral Ischemia. This is for better clinical guidance, and also for an effective, convenient and easy-going method and research thought of such kind of diseaseThis Dissertation summarizes the following two contents:one is to narrate the recognition of Mongolian medicine to Ischemic Cerebrovascular, to summarize the therapy condition of Mongolian medicine about Brain-blood diseases, and the literature review of the experiment research in the past20years; The other is the experiment report on the functions and mechanisms of Medical Warm Acupuncture for the Early Brain Damage by the24h Cerebral Ischemia Reperfusion Injury of the Rat. The experiment reports are as follows: Aim:observe the effect of Mongolian Medical Warm Acupuncture for Brain Damage after24hours by the Cerebral Ischemia Reperfusion Injury of the Rat, and discuss the function mechanisms of Mongolian Warm Acupuncture for the urgent Brain edema as well as the damage of the neural function.Method:Divide the SD male rat (weight280±20g) into the following five groups randomly:Model Group (M). False Operation Group (F), Warm Acupuncture Group (W). Conventional Acupuncture Group (C). Drug Contrastive Group (D). Use MCAO with the improved line plug method and go on reperfusion90min after the Ischemia. Treating method:treat1hour after the rat woke. Group W: choose Oroil Beqir, Hein Beqir and pierce the silver needle into them, them connect the electronic needle Therapeutic Apparatus, and keep the needle for30minutes; Group C, choose the acupuncture points of the big spine, air sea and Aminsodar. The Filoform needle pierces directly into them5mm,2mm and4mm, and it also needs keeping the needle30minutes. During the needle keeping, pierce the needle once in10minutes. Group D. fill the stomach once in accordance with the amount of10.8mg/ke of Nimodipine. Do the perfusion24hours after the Cerebral Ischemia of the rat, and choose the materials to do further observation to the experiment:(1) observe the score changes of the neural function evaluation for all the rats;(2) do the brain slice TTC staining, and after its firmation, deal with the brain slices of the rat with the Imaging System Software, and determinate the infarction volume of left brain damage;(3) do HE staining of the rat’s brain organization and observe the morphology change of the brain organization;(4) examine the rat’s serum and the contents change of MDA、 SOD、GSH、IL-1β、 IL-8、TNF-a、NO、NOS and ET-1with the method of ELISA;(5) Examine the content change of Hippocampus GFAP, NSE with the method of ELISA.Results:Among Group F and Group M, except for the special explanation of P>0.05, both have evident difference. This indicates that the contents between Ischemia and Non-Ischemia have big differences.(1) Neural function scores: compared with Group M, Pw, Pc<0.05; compared with Group C, Pw<0.05. Group W and Group C improve the neural function evidently, and Group W is far better than Group C.(2) Infarction volume of the brain: compared with Group M, Pc <0.05, Pw<0.01; Compared with Group D, Pw, Pc<0.05. The Infarction volume of the brain of Group W and Group C is clearly smaller than that of Group M and Group D.(3) Observation of the pathological section of HE staining:compared with Group M, the swelling and edema of the Nerve Cells of Group W, Group C and Group D are reduced, the cells of Group W does not sparse evidently and the neurons amount are increased.(4) MDA: compared with Group F, Pm, Pc, Pd>0.05, The content of serum MDA among all groups has no significant difference. But the mean value bar graph shows that the mean value of Group W is lower than that of Group M. SOD:compared with Group M, Pd, Pw<0.01. Pc<0.05; The content of serum SOD of Group W, Group D and Group C is higher than that of Group M. GSH:compared with Group M, Pw, Pc<0.05. The content of serum GSH of Group W and Group C is clearly higher than that of Group M. IL-8:compared with Group M, Pw, Pd<0.05. The content of serum IL-8and TNF-a of Group M and Group D is clearly less than that of Group M. NO, NOS:compared with Group M, Pd<0.05, Pw, Pc<0.01; The content of serum NO, NOS of Group W, Group D and Group C is clearly less than that of Group M, and Group W and Group C are far more significant. ET-1: compared with Group M, Pd<0.01, Pw, Pc<0.05. The content of serum ET-1of Group D, Group and Group C is evidently less than that of Group M, and Group D is the most significant. IL-1β, compared with Group M, Pw, Pc, Pd>0.05; Compared with Group F, Pm, Pc, Pd>0.05; The content of serum IL-1β among all Groups has no significant difference.(5) Organization of Hippocampus NSE:compared with Group M, Pw<0.01; The content of Hippocampus NSE is clearly less than that of Group M. GFAP:compared with Group M, Pw, Pc, Pd>0.05; Compared with Group F, Pm<0.05. The content of Hippocampus GFAP of Group D, Group W and Group C has no significant difference, but Group F and Group M have clear difference.Conclusion:(1) Mongolian Medical Warm Acupuncture can reduce evidently the defect score of the rat’s neural function by Cerebral Ischemic Reperfusion, and can improve the neural function and reveal the protective function of the Mongolian Medical Warm Acupuncture therapy from the aspect of neuroethology.(2) Mongolian Medical Warm Acupuncture has the function to reduce the volume of brain infarction and brain edema by observing TTC staining slice and HE pathological section, thus it can protect the continuous damage of the half dark bands in the early times of Cerebral Ischemic Reperfusion by reducing brain edema and narrowing the volume of brain infarction. It can also save and protect the injured neurons, and improve the treating role of the neural function.(3) By observing and evaluating the content of serum MDA, SOD, GSH, NO, NOS, ET-1in the early times of Cerebral Ischemic Reperfusion, it can be concluded that Mongolian Medical Warm Acupuncture has the tendency of anti-oxidize and anti-free radical damage to Cerebral Ischemic Reperfusion in its early period. Its mechanism maybe lower serum NO, NOS and ET-1, and increase serum SOD and GSH in the early period of Cerebral Ischemic Reperfusion. In this way, it can protect the free radical damage effectively and increase the anti-oxidize ability of brain tissue.(4) By observing and evaluating the content of serum TNF-a. IL-1β and IL-8, it can be concluded that Mongolian Medical Warm Acupuncture has the function of anti-inflammatory to Cerebral Ischemic Reperfusion in its early period. Its mechanism maybe reduce serum TNF-a and IL-8, and increase serum SOD and GSH in the early period of Cerebral Ischemic Reperfusion to achieve the role of anti-inflammatory and brain-protection effectively.(5) NSE can reflect the degree of brain damage in the early period of Cerebral Ischemic and act as one of the objective Biochemical indicators to the recovery degree of neural function recovery period. The research results indicate initially that Mongolian Medical Acupuncture has the effect of reducing the content of Hippocampus NSE in the early period of Cerebral Ischemic Reperfusion. This may become one of the reasons for why Mongolian Medical Acupuncture can save and protect the Ischemic Neurons.