The Role Of β1Integrin In The NVM Remodeling Response To Cerebral Ischemia Reperfusion Injury

Objective1. To evaluate the effects of β1integrin on the function of blood brain barrier in ischemia penumbra area and the animal ethology after brain ischemia reperfusion injury.2. To explore the neuroprotection effect of β1integrin on neurovascular unit (NVU) after brain ischemia reperfusion injury.Methods1. Calculate and analyse scores of MCAO rat’s synthesized neurological severity.2. Through the changes of apparent diffusion coefficient (ADC) of ischemic penumbra at different time points, we can evaluate the effect of β1integrin on the permeability of blood brain barrier.3. After injection of β1integrin antagonist to MCAO rats, we detect the expression and fluorescence intensity changes of neovascularization specific marker-VEGFR2and tight junction proteins claudin-5, ZO-1,which are important components of blood brain barrier(BBB).Results1. The scores of rat synthesized neurological severity of β1integrin antagonist injection group were higher than that of the model group at1day/4day/7day (P<0.01), which suggests the former group have more serious neural function defects.2. There is no obvious difference between the lday/4day/7day’s ADC value of β1integrin antagonist injection group and the model group (P>0.05), but the former is lower at14day (P<0.01)3. The expression of β1integrin of the antagonist injection group is obvious lower than that of model group at4day/7day (P<0.01). The fluorescence intensity and expression of VEGFR2in the former group are much lower than that in model group at1day/4day (P<0.01). The fluorescence intensity and expression of tight junction proteins claudin-5and zo-1in the former are also obvious lower than that in model group at lday/4day/7day/14day (P<0.01)Conclusion1. After cerebral ischemia reperfusion injury, block of β1integrin can inhibit recovery of blood brain barrier function of the ischemia penumbra.2. β1integrin plays an important role in repair and protection of neurovascular unit by promoting angiogenesis and up-regulating the expression of tight junction proteins.