| Research purposeResearch purpose of this experiment research objective:to study the health the preventive piece in the treatment of TCM and nonsteroidal anti-inflammatory drugs (nsaids) caused by acute gastric mucosa injury of curative effect, and to explore the prevention and control of party nonsteroidal related mechanism of action of stomach trouble, to lay a good foundation to further research in the future.Research methodAnimal research method is the first step:grouping:110will SPF Wistar rats, male and female50,110rats randomly divided into6groups, normal control group10, high and low dose group, model group, dangshen health in the preventive piece of high and low dose group of20respectively, and the western medicine group (omeprazole)20.Step2:animal building and dosage:normal group give regular feeding, given the rest of the group to according to the different weight to the corresponding drugs, treatment for7days, such as the western medicine group was given omeprazole, dangshen group was given infusion of codonopsis pilosula, treatment7days began to make mould indomethacin, immediately each take half, rats were cut to stomach, the stomach mucosa damage, reagent for testing at the same time, the rest of the rats with former feeding way, adhere to the drug, to look again take7days after the stomach, detection of gastric mucosa. Step3:using western blot (western blot), each group taking250mg of gastric mucosa tissue protein, equal amounts of protein, sds-page electrophoresis, transferred to nitrocellulose membrane, closed, respectively, to join the I resistance (Santa Cruz, USA Biotech production):cox-2polyclonal antibody and rabbit polyclonal antibody against rat after CREB, membrane washing, join anti-HRP rabbit IgG, sheep showed specificity protein bands, grey value is measured by computer image analysis system, to determine protein content accordingly.Results1. The general observations and normal control group, high dose kin in the preventive group:rats spirit as before, activity, reaction is normal, appetite to eat no obvious change, thick hair luster, stool forming, not soft, black. Omeprazole group, low dose of health in the preventive group, high dose groups:codonopsis pilosula spirit is a bit poor, rats decreased appetite to eat, activity, reaction reduce earlier, lose luster hair density, defecate day2times, soft. Model group, low dose group of rats spiritual malaise, codonopsis pilosula unresponsive, reduce or even lazy, intake significantly reduced appetite, hair loss and different level with gloss lost, defecate2-4times/day, bad or paste.2. Western medicine group, the sweet arenaceous nourishing the stomach pill group, high dose of health care in the preventive group, low dose of health care in the preventive group is in turn:75.31%,44.43%,92.27%,65.50%. With the model group, western medicine group, the sweet arenaceous nourishing the stomach pills group compared with statistical significance (P<0.05). Seven days after ulcer inhibition percentage results respectively, western medicine group, the sweet arenaceous nourishing the stomach pill group, high dose of health care in the preventive group, low dose of health care in the preventive group is in turn:83.38%,55.68%,96.43%,55.68%, and the model group, western medicine group, the sweet arenaceous nourishing the stomach pills group compared with statistical significance (P<0.05).3. Stomach tissues in the different groups including gastric mucosa nf-kappa beta expression increased, cox-2expression and PGE2synthesis increased compared with model group, normal group average (2.776+/-0.16), model group average (1.436+/-0.12), the western medicine group average (1.823+/-0.12), dangshen high-dose group average (2.139+0.16) dangshen low-dose group average (1.423+0.16) for health in the preventive group and high dose an average for (2.422+0.18) for health in the preventive group, low dose an average (2.039-0.18). With the model group, western medicine group, dangshen compared statistically significant (P<0.05).Conclusionthe health of the preventive piece of stomach trouble have correlation with nonsteroidal curative effect. The prescription by raising the rat gastric mucosa tissues in the central oxidase and the content of prostaglandin, promote gastric mucus secretion, improve gastric mucosal repair function, reduce the release of inflammatory mediators, and can reduce the stomach tissue, the content of gastric mucosa nf-kappa beta in reducing blood vessels within the physical activity of gastric mucosal injury, promote microcirculation, and from two aspects of play to the role of the protection and treatment of gastric mucosal tissue, embodies the advantages of Chinese medicine to treat disease. |
PDF Full Text Request