| Introduction High-grade gliomas are the most common and most malignant primary brain tumors in adults. Despite recent advances in surgery, radiation and chemotherapy, patients with high-grade gliomas have an overall poor outcome and will ultimately die of the disease. According to current World Health Organization (WHO) guidelines, gliomas are subcategorized by histopathologic evaluation into four tumor grades (â… -â…£), where glioblastoma (grade IV) is the most malignant and most common primary brain tumor. The prognosis is poor, showing a median survival of14.6months, a median progression-free survival of6.9months and a9.8%5year survival.Recent research suggests that deregulation of a group of noncoding RNAs (ncRNAs) called microRNAs (miRNAs) is involved in initiation and progression of many cancers including glioblastomas and that miRNAs hold great potential as future diagnostic and therapeutic tools.Several of the deregulated miRNAs are cancer-specific and often target key gene products involved in carcinogenesis, invasion and anti-apoptosis of the particular cancer of interest. The great potential of miRNAs is also reflected in the ability of a single miRNA to affect numerous targets. Several studies have compared miRNA expression in glioblastomas with different sorts of normal brain tissue (tissue adjacent to tumor or various non-neoplastic fetal or adult brain tissues). Both oncogenic and tumorsuppressive miRNAs were found to affect target genes involved in cell migration, cytoskeletal rearrangement, invasiveness, and angiogenesis. Clearly, the distinct functional properties of these miRNAs need further investigation and might hold a great potential in future molecular therapies targeting GBM.Objective To find out the expression profiles of MiRNAs in different histological type of glioma, and find new MiRNAs related to glioma; To detect the expression of MiR-125a-5p in different histological type of glioma samples, and the relationship between the expression of MiR-125a-5p and the invasiveness of GBM cells was confirmed in vitro experiment; Forecast their possible target genes by application of bioinformatics methods.Methods We Analysis the glioma database of CGGA, and find out the expression profiles of MiRNAs in different histological type of glioma. Using qRT-PCR experiments to verify the expression levels of MiR-125a-5p/MiR-128/MiR-7-1*in glioma samples. MTT experiments and transwell invasion assay were used to detect the changes of cell proliferation within96hours and the cell invasive ability after transfeting MiR-125a-5p mimics; Forecast the possible target genes of MiRNAs relative to the invasiveness of breast cancer cell by application of bioinformatics methods.Results:(1)42MiRNAs of differential expression were obtained from CGGA database, including35up-regulated MiRNAs and7down-regulated MiRNAs (2) Compared with normal brain tissue, qRT-PCR results showed that the expression levels of MiR-125a-5p in AA/AOA/AO/GBM are rising (3) Compared with the control group, the changes of GBM cell proliferation activity after transfecting MiR-125a-5p mimics were no significant within96hours (P>0.05).(4) Comparing to transfecting NC and the blank group, the invasiveness of GBM8401cell was apparently enforced by transfeting MiR-125a-5p mimics(P<0.01);(5) MiR-125a-5p has multiple target genes, MiR-125a-5p may be involved in regulating cell growth, proliferation, differentiation, apoptosis, transcription, signal transduction and other processes.Conclusion (1) MiRNAs expression profiles relative to different histological type was obtained, and MiR-125a-5p have much more expression level in AA/AOA/AO/GBM than A/O and normal brain tissue;(2) MiR-125a-5p had no effect on GBM cell proliferation activity;(3) MiR-125a-5p can inhibit the invasion of GBM cells. |
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