Study On The Mechanism Underlying The Treatmenton Functional Dyspepsia By Weikangning

Functional dyspepsia (Functional Dyspepsia, FD) refers to symptoms originating from the stomach-duodenum, and deficiency can cause the symptoms of organizational structure or biochemical abnormalities in a group of clinical syndrome. The etiology and pathogenesis of FD are a inconclusive, and researchers are looking for effective treatment options. Traditional Chinese Medicine treatment for FD is effective, and has become a major research hotspot of medicine in treating FD. The therapy of Acrid openning and Bitter Descending is used to adjust the function of Qi, conformed to the stiology and pathogenesis of FD, WeiKangNing(WKN) is representative prescription guided by this theory, which has been used as treatment for FD for over 20 years and proved both effective and safe, but how dose it work needs our study and discuss.Early laboratory research indicates that WKN can regulate the contents of GSTP、SOD2、 VDAC1、ENO1 in FD model rat’s gastric tissue. VDAC1 and ENO1 are cital biotic factors participating in the progress of energy metabolism, GSTP and SOD2 are significant biological indicators in Redox. We guess that WKN may play a critical role in treating FD by affecting both energy metabolism and redox in cells. Autophagy is a hot sport of research and it also can generate FD. Therefore we also studied autophagy in our research.In vivo tests, we regarded GATP、SOD2、MDA as indexes in redox, VDAC1、ENO1、ATP in energy metabolism, and measure LC3 to assess autopyagy based on these indicators we explored the mechanism of WKN. We activated gastric eprthetial cells and chose Domperidone as positive control carried outcytotoxicity assays by CCK8, confirmed the administration and concentration of WKN in energy metabolism、redox as well as autophagy. WKN can promote the expression of GSTP and SOD2 in gastric epithelial cells in addition by Western Blot and RT-PCR, reduce the content of MDA by TBA. There was no evidence showed that WKN changed energy metabolism and autophagy situation. Results above indicate that the drug has specific function in redox, so antiosidant may be an important point in phatmaceutical mdchanism. To verify the results, we continue to carried out in vivo tests, we preparedf functional dyspepsia rats, to detect influence of MDA content in gastric Corning gastric tissue in rats.We can know from the consequence that the contend of MDA in control group rose apparently while the content of MDA in rats give WKN showed a tendency of decline. In order to study the reliability of WKN in actioxidant, Having knocked down and over expression GSTP and SOD2 in GSE-1 resoectively. We checked the influence of WKN to the content of MDA in GSE-1 the result showed that compared with blank group the content of MDA visibly in over expressed GSTP and SOD2.This find demonstrates that WKN can antioxidant indeed, which may be the mechanism of treating FD.In general, the research studied the explore of Redox、Energy Metabolism and Autophagy, the results show that WKN has specific function in Redox, the anti oxidation of medicine can adjust the oxidative stress in cells, which has been proved by knocked down and over expression. These researches deepen our understanding on WKN’s test to FD, at the same time, it implies that the oxidativestress injury of gasiric epithecial cell may be one of the factors leading to FD. In addition, WKN has better regulating effect than GSTP, which can provide thought to centralized research on WKN’s antioxidation in treatment of FD.