The Experimental And Clinical Research On Huoxue Huayu Herbs Intervene Tissue Regeneration After Microsurgery Based On The Theory Of "Qu Yu Sheng Xin"

Experimental ResearchPart1The experimental research on Fu Yuan Huo Xue decotion intervene Rat Femoral artery injury "Xue-Mai-Wu"Cascade regeneration after microsurgery based on the theory of "Qu Yu Sheng Xin"Objective:Explore the"Qu Yu Sheng Xin"theory and its representatives prescription Fu Yuan Huo Xue decoction therapy guidance in microsurgical v ascular injury and repair postoperative.Assess its effectiveness intervention on postoperative vascular injury"Xue,Mai,Wu"cascade newborn.Mining the theory connotation and denotation in microsurgery field.Methods:96SD rats were randomly divided into four groups,prepare th e animal models which cutting the femoral artery and anastomosis. Each grou ps interventions were saline Fu Yuan Huo Xue decoction,Dextran&papaveri ne and three-drug combination,then observe the results at point of24hours,3days,7days,15days.From the"Yu remove"to"Xue,Mai,Wu"three angles assess the efficacy of tissue repair and newborn in postoperative.By blood rheology,6-Keto-PGFia,TXB2determination to evaluate the changes of blood flow,scilice t"Xue newborn".By real-time quantitative PCR vascular tissue VEGF,Flk-l,An g-1,Tiel mRNA expression,morphology and ultrastructure changes in optical microscopy and SEM,TEM to evaluate the efficacy of vascular repair newbor n,scilicet"Mai newborn".With targeted muscle tissue serum SOD and MDA co ntent to evaluate microcirculation changes in the corresponding structure,scilic eT“Wu newborn"and concatenation. Integrated three aspects of tissue repair ne wborn to summarize analytical"Qu Yu Sheng Xin" theory of meaning in micr osurgery.Result; After microsurgical vascular repair,the FYHX decoction group bl ood rheology,6-K.eto-PGF1α,TXB2content in terms of efficacy and the compari son group rather were significantly better than the control group (p<0.05),an d"Xue newborn"indicators are more superior than Dextran&papaverine grou p.FYHXT group or combination group the vascular tissue VEGF, Flk-1, Ang-1, Tiel mRNA expression,scilicet"Mai newborn"was significantly better than t he two control groups (p<0.05), observation of vascular tissue morphology i n the light microscope and SEM, TEM ultrastructural changes compared with the control group was also excellent.Combination group compared with the s imple application of Traditional Chinese Medicine in terms of morphology an d ultrastructure was excellent,quantitative microscopy observations suggest the presence of electric statistically significant (p<0.05).In the early postoperativ e, no significant late (p>0.05) in the late postoperative;Each time the targete d muscle tissue SOD activity,MDA concentration CH group and SPSS group were statistically significant(p<0.05).CH group is better than the control grou p.On comprehensive assessmen"Qu Yu Sheng Xin"theory and its representativ es recipe FYHX decoction interventions have a positive effect in terms of rat vascular anastomosis newborn.More in-depth discussion of its significance tre atment can be performed by clinical research.Experimental ResearchPart2The experimental research on Fu Yuan Huo Xue decotion intervene Rat Sciatic nerve injury "Xue-Mai-Wu"Cascade regeneration and nerve repair after microsurgery based on the theory of "Qu Yu Sheng Xin" Objective:Explore the"Qu Yu Sheng Xin"theory and its representatives prescription Fu Yuan Huo Xue decoction therapy guidance in microsurgical p eripheral nerve injury and repair postoperative.Assess its effectiveness interven tion on postoperative vascular injury"Xue,Mai,Wu" cascade newborn and The efficacy of nerve repair.Mining the theory connotation and denotation in micr osurgery field.Methods:120SD rats were randomly divided into four groups,prepare t he animal models which cutting the Sciatic nerve and anastomosis. Each gro ups interventions were saline,Fu Yuan Huo Xue decoction,Mecobalamin by ga vage(ig),then observe the results at point of lweek,4weeks,8weeks,12weeks.Fr om the"Yu remove"to"Xue,Mai,Wu"three angles assess the efficacy of tissue r epair and newborn in postoperative.By serum EPO,nerve tissueCD34,VEGF pr otein expression to confirmed Fu yuan Huo xue decoction can promote blood, pulse newborn after microsurgery effectively.Scilicet "QuYuShengXin" theory has the effect on angiogenesis and microcirculation improving nerve regenerat ion environment after peripheral nerve on microsurgery anastomosis.By FG fl uorescent tracer,neurotrophic factor like BDNF、Par-3、 P0、S100mRNA deter mination,NGF, collagen westernblot observation and SEM TEM to evaluate th e efficacy on peripheral nerve repair newborn combined with general observa tion,SFI,EMGDiscussion Fu Yuan Huo Xue decoction accelerate repair of neu rogenesis based on "Xue Mai newborn",.By targeting muscle Histological mor phological changes, to analysis "Qu Yu Sheng Xin"theory and angiogenesis, nerve regeneration,targeted muscle newborn scilicet"Xue,Mai,Wu" cascade new born association.Result:After microsurgical peripheral nerve repair,the FYHX decoction g roup EPO、VEGF、CD34were significantly better than the two control group (p<0.05),and"Xue Mai newborn"indicators were best superior.Fuyuan Huoxu e decoction group gold retrograde fluorescent tracer, BDNF, Par-3, P0, S100mRNA expression assay respects considerable effect Mecobalamin group (p>0.05),and more excellent measurement results in SFI,EMG more better than M ecobalamin group,(p<0.05).NGF, collagen westernblot observation and adhesio n scar analysis demonstrate that there was significant difference (p<0.05) bet ween thethree groups.On comprehensive assessment"Qu Yu Sheng Xin"theory and its representatives recipe FYHX decoction interventions have a positive e ffect in terms of rat peripheral nerve repair anastomosis newborn. More in-de pth discussion of its significance treatment can be performed by clinical resea rch.Clinical ResearchPart1Fu Yuan Huo Xue decoction intervene survival and functional recovery after replantation surgery based on the theory of "Qu Yu Sheng Xin"Objective:Assessment "Qu Yu Sheng Xin"theory and its representatives prescription Fu Yuan Huo Xue decoction in microsurgery finger replantation surgery guiding significance, explore the "Xue,Mai,Wu"cascade newborn and r eplantation survival,function treatment effect in postoperative. The theory is f urther revealed connotation and denotation from the clinic in the field of mic rosurgery.Methods:The60cases of replantation surgery were randomly divided i nto treatment group and control group,30cases of patients in each group.Aft er replantation surgery,based on early anticoagulation antispasmodic drugs,patie nts treated with oral FYHX decoction for postoperative treatment in whole co urses.By early blood rheology,coagulation determination and survival of levels, a comprehensive assessment of early survival situation.Withing postoperative f ollow-up at different time points,by X-ray,microcirculation examination,functio nal index rating quantitative score,compare the functional recovery in postoper ative.Assessment verification"Qu Yu Sheng Xin"theory and FYHX efficacy to replantation by "Xue,Mai,Wu"cascade newborn after each stage of treatment. Further analysis of "Qu Yu Sheng Xin"theoretical connotation on replantation clinical research.Result:The FYHX decoction group the early blood rheology,coagulation determination and survival of levels were significantly better than the control group (p<0.05) in postoperation,application of Chinese Medicine in the earl y"Xue,Mai newborn" and replanted fingers survived more excellent effect. FY HX decoction group X-ray,microcirculation examination,functional index rating was significantly better than the control groups (p<0.05) on postoperative f ollow-up. Application of Chinese Medicine in the late"Mai,Wu newborn" and replanted fingers repair and functional recovery have more excellent effect.C omprehensive assessment"Qu Yu Sheng Xin"theory and its representatives reci pe FYHX decoction interventions have a positive effect in replantation efficac y.Clinical ResearchPart2Fu Yuan Huo Xue decoction intervene survival and functional recovery after bone-skin flap Graft surgery based on the theory of "Qu Yu Sheng Xin"Objective:Assessment"Qu Yu Sheng Xin"theory and its representatives p rescription Fu Yuan Huo Xue decoction in microsurgery bone-skin flap graft guiding significance, explore the "Xue,Mai,Wu"cascade newborn and replantati on survival,function,repair and regeneration treatment effect in postoperative.T he theory is further revealed connotation and denotation from the clinic in th e field of microsurgery.Methods:The40cases of bone-skin flap graft were randomly divided i nto treatment group and control group,20cases of patients in each group.Afte r operation,based on early anticoagulation antispasmodic drugs,patients treated with oral FYHX decoction for postoperative treatment in whole courses.By ea rly blood rheology,coagulation determination,survival of levels,donor site graft survival and peripheral circulation etc.Comprehensive assessment of bone-skin flap early survival and donor site healing situation.Withing Postoperative foll ow-up at different time points,by X-ray,microcirculation examination,bilateral li mb peripheral circulation,muscle strength,wound healing,tissue flap feeling and microcirculation compare the the flap and donor site recovery in postoperati ve.Assessment verification"Qu Yu Sheng Xin"theory and FYHX decoction effi cacy to bone-skin flap graft by "Xue,Mai,Wu" cascade newborn after each st age of treatment.Further analysis of Qu Yu Sheng Xin"theoretical connotation on tissue flap clinical research.Result:The FYHXT group the early blood rheology,coagulation determin ation,survival of levels,donor site graft survival and peripheral circulation etc. were significantly better than the control group (p<0.05) in postoperation.A pplication of Chinese Medicine in the early donor site healing situation and t issue flap survived have more excellent effect.FYHX decoction group X-ray,m icrocirculation examination,bilateral limb peripheral circulation,muscle strength, wound healing,tissue flap feeling and microcirculation were significantly better than the control groups (p<0.05) on postoperative follow-up.Application of Chinese Medicine in the late"Xue,Mai,Wu newborn" and the flap and donor site recovery more excellent effect.Comprehensive assessment"Qu Yu Sheng X in"theory and its representatives recipe FYHX decoction interventions have a positive effect in bone-skin flap graft efficacy.