The Research On Mast Cell-Mediated Effect Mechanism Of Navel Therapy Of Treating Diarrhea-predominant Irritable Bowel Syndrome

Objective:1.To verify the effectiveness of navel therapy of treating diarrhea-predominantirritable bowel syndrome(IBS-D).2.To discuss about the relationship between the effect of navel therapy and mastcell(MC) in acupoint area and target organ through observing the influence of naveltherapy to the number of mast cells and expression of Toll-like receptors(TLRs), transientreceptor potential vanilloid(TRPV) in Shenque(CV8) and colon of rats with IBS-D, andthen to explain the effect mechanism of navel therapy of treating IBS-D from the angle ofmast cell.Methods:1.The rats were randomly divided into5groups named the normal group, the modelgroup, the group of herb-partition moxibustion on the navel, the group of drug coveringnavel therapy and the group of navel therapy with moxa stick moxibustion. In addition tothe normal group, the other4groups were set up the model of IBS-D. After the success ofthe model, the normal group and the model group were only grabbed and fixed as the sameas the rest groups but without any treatment; the other groups were respectively treated byherb-partition moxibustion on the navel, drug covering navel therapy and navel therapywith moxa stick moxibustion.10days after the start of treatment, these groups wereassessed and detected.2.We observed the symptoms, colon movement and intestinal sensitivity includingmental state, fur burnish, diet, growth rate of body weight, condition defecation, fecalcharacter, water content of fecal, abdominal withdrawal reflex and so on and then compared the differences among these groups.3.We used methods such as modified toluidine blue staining andimmunohistochemistry to test the number of mast cells and expression of Toll-like receptor2(TLR2), Toll-like receptor4(TLR4) of TLRs and transient receptor potential vanilloid1(TRPV1), transient receptor potential vanilloid2(TRPV2)and transient receptor potentialvanilloid4(TRPV4) of TRPV in Shenque(CV8) and colon of the rats, and then comparethe difference among these groups.Results:1.The model rats of IBS-D have worse changes in terms of improvement than healthyrats in symptoms, colon movement and intestinal sensitivity. In terms of the above effectindicators, the group of herb-partition moxibustion on the navel, the group of drugcovering navel therapy and the group of navel therapy with moxa stick moxibustion are allsuperior to the model group(P<0.05), and the group of herb-partition moxibustion on thenavel is superior to the group of drug covering navel therapy and the group of naveltherapy with moxa stick moxibustion(P<0.05).2.In terms of the number of mast cells and expression of TLR2, TLR4, TRPV1andTRPV4in Shenque(CV8), the group of herb-partition moxibustion on the navel, the groupof drug covering navel therapy and the group of navel therapy with moxa stickmoxibustion are all higher than the model group(P<0.05), and the group of herb-partitionmoxibustion on the navel is higher than the group of drug covering navel therapy and thegroup of navel therapy with moxa stick moxibustion(P<0.05); in terms of the number ofTRPV2in Shenque(CV8), the group of herb-partition moxibustion on the navel, the groupof drug covering navel therapy and the group of navel therapy with moxa stickmoxibustion are all higher than the model group(P<0.05), and the group of herb-partitionmoxibustion on the navel and the group of navel therapy with moxa stick moxibustion arehigher than the group of drug covering navel therapy(P<0.05); in terms of the aboveindicators in colon, the group of herb-partition moxibustion on the navel, the group of drugcovering navel therapy and the group of navel therapy with moxa stick moxibustion are alllower than the model group(P<0.05), and the group of herb-partition moxibustion on thenavel is lower than the group of drug covering navel therapy and the group of naveltherapy with moxa stick moxibustion(P<0.05).Conclusion:1.Herb-partition moxibustion on the navel, drug covering navel therapy and navel therapy with moxa stick moxibustion can improve the symptoms, colon movement andintestinal sensitivity of the rats with IBS-D.2.Herb-partition moxibustion on the navel, drug covering navel therapy and naveltherapy with moxa stick moxibustion can enhance the number of mast cells and theexpression of TLRs and TRPV receptors in the acupoint area.3.Herb-partition moxibustion on the navel, drug covering navel therapy and naveltherapy with moxa stick moxibustion can reduce the number of mast cells and theexpression of TLRs and TRPV receptors in target.organ.4.The advantages of herb-partition moxibustion on the navel are more obvious.