| Objective：To explore the brain functional changes of the patients with over-anxietycondition (OAC) in resting state, to present the relationship between OAC and abnormalbrain function,to supply the objective method for OAC in modern research and cliniceffective evaluation.Methods：The subjects of this study were40master’s degree students and doctoralstudents from Shandong University of Traditional Chinese Medicine matched with age andgender including20over-anxiety patients and20healthy volunteers.Resting-statefunctional MRI (RS-fMRI) scan were received in October to November of2013tocompare and analyze their brain activities with the regional homogeneity (ReHo) andfunctional connectivity method in the resting state. The scale for over-anxiety condtion(OACS) was used to measure the severity of over-anxiety condition and then explored therelationship between ReHo and OACS.10subjects were chosen from the OAC group to receive second fMRI scan to measuretest-retest reliability with intraclass correlation coefficient (ICC) in the local Brodmannarea and the whole brain level.Results：1. Compared with healthy subjects, ReHo value of over-anxiety patients onthe left cerebellum in resting state reduced and ReHo value increased on the left medialtemporal lobe, the right gyrus subcallosum, the left posterior cingulate gyrus and the rightsuperior frontal gyrus. The statistic threshold was set at P <0.05, cluster connectivitycriterion5rmm with Alphasim correction. The ReHo had significantly positive correlation with the total score and Psychological behavior factor score of OACS, respectively (P＜0.05).2. Compared with healthy subjects, the left cerebellum of OAC patients showedincreased functional connectivity with the right parahippocampal gyrus, on the contrary,decreased functional connectivity to the left inferior parietal lobule; the right gyrussubcallosum showed increased functional connectivity with the left middle frontal gyrus;the right ventral Posterior cingulate cortex showed increased functional connectivity toparticular brain regions including the right fusiform gyrus, lingual gyrus, culmen ofcerebellum, cuneus and the left lingual gyrus, Middle occipital gyrus and precuneus (P＜0.01).The functional connectivity strength had no significant difference between OACpatients and healthy subjects. But in OAC group, the left cerebellum and the left medialsurface of the temporal lobe, the left cerebellum and the right gyrus subcallosum, the leftmedial surface of the temporal lobe and the right superior frontal gyrus,were all negativecorrelation, on the contrary, positive correlation in health group.3. ICCs were less than0.2in BA11, BA17, BA32, BA43, BA46, BA48, which wereconsidered no correlation; ICCs were between0.2and0.4inBA1,BA4,BA5,BA6,BA20,BA23,BA25,BA26,BA27,BA29,BA37,BA40,BA41,BA44，which meant fair test-retest reliability; ICCs were between0.4and0.6in BA1,BA19,BA38,BA39,BA40, which meant moderate test-retest reliability; ICCs were between0.6and0.8in BA2,BA3,BA8,BA9,BA21,BA22,BA24,BA45, which meant substantialtest-retest reliability; ICC of all the Brodmann area was0.921, reached perfect level oftest-retest reliability. ICC of the whole brain was0.746, meant substantial test-retestreliability.Conclusions:1. On the resting state, brain functions of over-anxiety patients areabnormal. These regions are affiliated with mood, cognition and gastrointestinalfunction, so over-anxiety patients have potential risk of cognitive impairment.2. Partial abnormal brain function is earlier than the whole functional connectivity, thelatter may accompany cognitive impairment.3. Dysfunction of neural network which consist of cerebellum and multiple brainregions of limbic system may be the reason of abnormal brain function of OAC patients. 4. The good test-retest reliability display the RS-fMRI can reflect the true brainfunction of OAS OAC patients. Simultaneously, it also illustrates reliability and accuracyof the first fMRI scan. |
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