Comparative Proteome Study On Ovarian Failure And The Differential Expression Of The Related Proteins

Premature menopause affects women and results in infertility.The mechanismsfor folliculogenesis and ovarian function have been widely studied. we investigateddifferential protein expression in the ovaries of menopausal versus childbearingwomen using DIGE and MS. We identified four differentially expressed proteins bywestern blot and immunohistochemistry (IHC).There were31differential expressioned proteinum in the ovaries of menopausalwomen，14proteinum were up-regulated，17proteinum were down-regulated. Thefour proteins were identified as functional proteins: serum amyloid P(SAP), heatshock protein27(HSP27), Glyoxalase I (GLO I)and Ubiquitin carboxy-terminalhydrolase L1(UCH-L1). All four proteins were up-regulated in the ovaries ofmenopausal women, and they were predominantly expressed in the atretic follicles ofmenopausal women, and had an altered expression in the follicles of differentdeveloped phase. SAP were clearly observed in the cells of the different developedphase follicles. HSP27were higher observed in the oocyte of primordial follicles andprimary follicles and the atretic follicles.GLO I were higher observed in the granulosaand theca cells of the secondary follicles and mature follicles，and highest observed inthe atretic follicles. UCH-L1were higher observed in the granulosa and theca cells ofthe secondary follicles and mature follicles，and weak observed in the atretic follicles.All the proteins were observed in the cytoplasm of ovum cells. And the four proteinswere not observed in the corpus albicans.The first study on differential protein expression in the ovaries of menopausalwomen versus childbearing women.These data suggest that the four functionalproteins may play a role in the regulation of the follicle development and the follicleatresia.These data may provide an target for the therapy and prevention of thepremature menopause, or premature ovarian failure.